Emerging evidence suggests that growth factors are crucial in regenerative endodontic therapy. To achieve the desired effects, the systematic administration of supraphysiologic concentrations of exogenous growth factors is commonly performed, but this is usually associated with high costs, technique, and safety issues. Here, we describe a novel biomaterial that can manipulate endogenous growth factors without the need for adding exogenous growth factors. Transforming growth factor β1 binding peptide (TGFp) was grafted onto the surface of a neutral pH phytic acid–derived bioactive glass (PSC) to synthesize modified bioactive glass (PSC-TGFp). Fourier transform infrared spectroscopy and thermogravimetric analysis results demonstrated that the TGFp was successfully grafted to the surface of the PSC. Scanning electron microscopy and x-ray diffraction showed that PSC-TGFp possessed good in vitro bioactivity. After soaking in simulated body fluid for 24 h, hydroxyapatite formed on the surface of PSC-TGFp. Enzyme-linked immunosorbent assay showed that PSC-TGFp could capture endogenous transforming growth factor β1 from dentin matrix–extracted proteins (DMEP) and release it slowly over 21 d. Cytologic experiments revealed that PSC-TGFp after adsorbing DMEP could enhance the adhesion, migration, viability, and odontogenic differentiation of stem cells from apical papilla. The results highlight that PSC-TGFp may be a promising biomaterial to manipulate endogenous growth factors for regenerative endodontic therapy in the future.

新出现的证据表明，生长因子在再生牙髓治疗中至关重要。为了达到预期的效果，通常会系统地施用超生理浓度的外源性生长因子，但这通常与高成本、技术和安全问题相关。在这里，我们描述了一种无需添加外源性生长因子即可操纵内源性生长因子的新型生物材料。将转化生长因子β1结合肽（TGFp）移植到中性pH植酸衍生的生物活性玻璃（PSC）表面，合成改性生物活性玻璃（PSC-TGFp）。傅里叶变换红外光谱和热重分析结果表明TGFp成功接枝到PSC表面。扫描电镜和X射线衍射表明PSC-TGFp具有良好的体外生物活性。在模拟体液中浸泡24 h后，PSC-TGFp表面形成羟基磷灰石。酶联免疫吸附试验表明，PSC-TGFp 可以从牙本质基质提取蛋白 (DMEP) 中捕获内源性转化生长因子 β1，并在 21 d 内缓慢释放。细胞学实验表明，吸附 DMEP 后的 PSC-TGFp 可以增强根尖乳头干细胞的粘附、迁移、活力和成牙本质分化。结果突出表明，PSC-TGFp 可能是一种有前途的生物材料，可在未来操纵内源性生长因子用于再生牙髓治疗。在 PSC-TGFp 表面形成羟基磷灰石。酶联免疫吸附试验表明，PSC-TGFp 可以从牙本质基质提取蛋白 (DMEP) 中捕获内源性转化生长因子 β1，并在 21 d 内缓慢释放。细胞学实验表明，吸附 DMEP 后的 PSC-TGFp 可以增强根尖乳头干细胞的粘附、迁移、活力和成牙本质分化。结果突出表明，PSC-TGFp 可能是一种有前途的生物材料，可在未来操纵内源性生长因子用于再生牙髓治疗。在 PSC-TGFp 表面形成羟基磷灰石。酶联免疫吸附试验表明，PSC-TGFp 可以从牙本质基质提取蛋白 (DMEP) 中捕获内源性转化生长因子 β1，并在 21 d 内缓慢释放。细胞学实验表明，吸附 DMEP 后的 PSC-TGFp 可以增强根尖乳头干细胞的粘附、迁移、活力和成牙本质分化。结果突出表明，PSC-TGFp 可能是一种有前途的生物材料，可在未来操纵内源性生长因子用于再生牙髓治疗。细胞学实验表明，吸附 DMEP 后的 PSC-TGFp 可以增强根尖乳头干细胞的粘附、迁移、活力和成牙本质分化。结果突出表明，PSC-TGFp 可能是一种有前途的生物材料，可在未来操纵内源性生长因子用于再生牙髓治疗。细胞学实验表明，吸附 DMEP 后的 PSC-TGFp 可以增强根尖乳头干细胞的粘附、迁移、活力和成牙本质分化。结果突出表明，PSC-TGFp 可能是一种有前途的生物材料，可在未来操纵内源性生长因子用于再生牙髓治疗。