A sensitive, simple, label-free electrochemical cytosensor was developed to evaluate the anticancer activity of pinoresinol against human cervical carcinoma (HeLa) cells. HeLa cells were immobilised on carboxylated multi-walled carbon nanotubes (c-MWCNTs)/gold nanoparticles (AuNPs) nanocomposite-modified glassy carbon electrodes. Scanning electron microscopy, transmission electron microscopy, Fourier transform-infrared spectroscopy, and X-ray diffractometry were used to characterise the morphology, crystallinity, and composition of the nanocomposites. Cyclic voltammetry was used to characterise and optimise the cytosensor. Both c-MWCNTs and AuNPs increased the electron-transfer rate between the HeLa cells and the electrode, retaining good cell compatibility. The concentration of HeLa cells immobilised on the nanocomposite electrode exhibited a good correlation with the impedance values determined by electrochemical impedance spectroscopy in the range of 10²–10⁶ cells/mL (detection limit of 10² cells/mL) with R² = 0.975. HeLa cells were then immobilised on the nanocomposite electrode at a concentration of 10⁴ cells/mL, and it was used as a cytosensor. The anticancer activity of pinoresinol from Cinnamomum camphora was evaluated by determining the median inhibitory concentration using the proposed cytosensor. For comparison, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and fluorescent imaging were simultaneously performed, which confirmed the results of the cytosensor. The cytosensor is promising for future applications in anticancer drug screening.

开发了一种灵敏、简单、无标记的电化学细胞传感器，用于评估松脂醇对人宫颈癌 (HeLa) 细胞的抗癌活性。HeLa 细胞被固定在羧化多壁碳纳米管 (c-MWCNTs)/金纳米粒子 (AuNPs) 纳米复合改性玻碳电极上。扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱和 X 射线衍射用于表征纳米复合材料的形态、结晶度和组成。循环伏安法用于表征和优化细胞传感器。c-MWCNTs和AuNPs都增加了HeLa细胞和电极之间的电子转移率，保持了良好的细胞相容性。² –10 ^{6 个}细胞/mL（检测限为 10 ^{2 个}细胞/mL），R ²  = 0.975。^{然后将HeLa细胞以10 4 个}细胞/mL的浓度固定在纳米复合电极上，并将其用作细胞传感器。通过使用所提出的细胞传感器确定中位抑制浓度来评估来自樟脑的松脂醇的抗癌活性。为了比较，同时进行了 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 测定、流式细胞术和荧光成像，这证实了细胞传感器的结果。该细胞传感器在未来抗癌药物筛选中的应用前景广阔。