Triploidy is induced in Atlantic salmon (Salmo salar) to produce sterile fish for genetic containment and to hinder early sexual maturation in farmed fish, but it can have unwanted negative effects on growth, health, and welfare. However, the growth and welfare of triploid fish may be improved by adjusting the rearing environment, feeding conditions and diets. This study evaluated physiological changes and used a suite of biomarkers to assess the potential impact of diet on growth and welfare of diploid and triploid salmon during the parr-smolt transformation. Diploids and triploids, held at low temperature, were fed a standard salmon feed or one with hydrolyzed fish proteins thought to be suitable for triploid Atlantic salmon. Fish muscle was collected monthly from October to December (2454–3044 degree-days post-start feeding, ddPSF) for analysis of biomarkers, and the progress of the parr-smolt transformation was monitored using a seawater challenge test. Real-Time PCR and radioimmunoassay were used to assess growth and stress response biomarkers (expression of genes of the GH-IGF axis and HSP70; cortisol concentrations), and oxidative stress biomarkers of lipids (MDA) and proteins (AOPP) were assayed. Changes in the biomarkers were related to sampling time rather than being associated with diet or ploidy, and the changes were compatible with the progression of the parr-smolt transformation. Growth and expressions of the biomarkers in triploid Atlantic salmon were similar to those of their diploid counterparts, and there was no evidence that the rearing conditions employed in the study resulted in stress responses being elicited. Overall, the physiological indicators and biomarkers employed in this study did not point to there being any dietary effects on performance and welfare of diploid and triploid salmon that were undergoing parr-smolt transformation.

三倍体在大西洋鲑鱼（Salmo salar) 生产用于遗传控制的不育鱼并阻碍养殖鱼的早期性成熟，但它可能对生长、健康和福利产生不利的负面影响。然而，通过调整饲养环境、饲养条件和饮食，可以改善三倍体鱼的生长和福利。这项研究评估了生理变化，并使用了一套生物标志物来评估饮食对parr-smolt转化过程中二倍体和三倍体鲑鱼生长和福利的潜在影响。在低温下保存的二倍体和三倍体被喂食标准鲑鱼饲料或一种被认为适合三倍体大西洋鲑鱼的水解鱼蛋白饲料。从 10 月到 12 月（开始喂食后 2454-3044 度日，ddPSF）每月收集鱼肌肉，用于分析生物标志物，并且使用海水挑战测试监测parr-smolt转化的进展。实时荧光定量 PCR 和放射免疫测定用于评估生长和应激反应生物标志物（GH-IGF 轴和 HSP70 基因的表达；皮质醇浓度），并测定了脂质 (MDA) 和蛋白质 (AOPP) 的氧化应激生物标志物。生物标志物的变化与采样时间有关，而不是与饮食或倍性有关，并且这些变化与 parr-smolt 转化的进展相一致。三倍体大西洋鲑鱼的生物标志物的生长和表达与其二倍体对应物相似，并且没有证据表明该研究中采用的饲养条件会导致应激反应。总体，