This study aimed to evaluate the effect of plant-based diets containing different inclusion levels (7%, 14%, 21% and 28%) of mixed culture microorganisms fermented soybean meal (FSBM) on the zootechnical performance and intestinal health of juvenile Nile tilapia (Oreochromis niloticus) reared in a biofloc system. The FSBM diets were compared with a positive control diet with fish meal and a negative control diet with no animal protein. All diets were isoprotein (33% CP) and isoenergetic (4300 kcal GE/kg). The design was completely randomized with four replications and the experiment lasted 54 days. Juveniles (1.635 ± 0.198 g) were distributed in 24 tanks (70 L) at a density of seven animals per tank. Fermentation increased probiotic microorganisms count (lactic acid bacteria 4.75 ± 0.21 log CFU g⁻¹ and yeast 3.30 ± 0.54 log CFU g⁻¹) and improved nutritional characteristics of FSBM. In fish fed the diet containing 7% FSBM, growth performance did not differ from fish fed the fish meal diet. FSBM inclusion improved the food efficiency of plant-based diets. The inclusions above 21% of FSBM increased the intestinal villi height. In the inclusion of 28% of FSBM, the goblet cell was higher compared to that of the other plant-based diets. The inclusion of 7% FSBM allows a total replacement of fish meal without compromising the growth performance of the animals.

中文翻译：

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混合培养微生物发酵豆粕提高了在生物絮团系统中饲喂植物性日粮的尼罗罗非鱼 (Oreochromis niloticus) 幼鱼的生产性能和肠道健康

本研究旨在评估含有不同含量（7%、14%、21% 和 28%）混合培养微生物发酵豆粕 (FSBM) 的植物性日粮对尼罗罗非鱼幼鱼养殖性能和肠道健康的影响( Oreochromis niloticus ) 在生物絮团系统中饲养。将 FSBM 饮食与含有鱼粉的阳性对照饮食和不含动物蛋白的阴性对照饮食进行比较。所有日粮均为异蛋白（33% CP）和等能（4300 kcal GE/kg）。该设计完全随机，重复四次，实验持续 54 天。幼鱼 (1.635 ± 0.198 g) 分布在 24 个水箱 (70 L) 中，每个水箱的密度为 7 只。发酵增加益生菌微生物数量（乳酸菌 4.75 ± 0.21 log CFU g^-1和酵母 3.30 ± 0.54 log CFU g ^-1 ) 并改善了 FSBM 的营养特性。饲喂含 7% FSBM 日粮的鱼的生长性能与饲喂鱼粉日粮的鱼没有差异。FSBM 的加入提高了植物性饮食的食物效率。超过 21% FSBM 的夹杂物增加了肠绒毛的高度。在加入 28% 的 FSBM 时，杯状细胞的含量高于其他植物性饮食。加入 7% FSBM 可以完全替代鱼粉，而不会影响动物的生长性能。