A novel technologically advanced fermented wheat gluten and yeast sourced protein (FWPC) co-product utilising Saccharomyces cerevivisiae yeast was evaluated for juvenile Nile tilapia Oreochromis niloticus. The protein component of a semi-purified basal diet (control treatment) was incrementally replaced by Fermented Wheat Protein Concentrate (FWPC) at 10%, 20%, and 30% of total dietary protein to create a slope-assay response to the test ingredient compared to an ‘ideal protein’ essential amino acid EAA balanced source. After an 8-week feeding trial, tilapia fed 10% and 20% FWPC displayed similar performance to those fed the control FM based diet, while fish fed 30% FWPC gave the lowest growth performance (P ≤ 0.05) compared to the other treatments. Proximate composition analysis showed that body protein, lipid, ash, NFE and gross energy were unaffected by dietary treatment (P ≥ 0.05). Both plasma alanine aminotranferase (ALAT) and aspartate aminotranferase (ASAT) were measured as indicators of hepatic function in Nile tilapia. No effects were seen in ALAT and ASAT activities. Tilapia fed 30% FWPC showed reduced HSI (P ≤ 0.05) compared to the other treatments. Additionally, histological comparisons of liver and intestinal tissues gave no indication of impairment to health, but FWPC inclusion elevated the number of goblet cells present in the posterior intestine (P ≤ 0.05). Ultrastructure of microvilli height and density also increased significantly showing functional aspects of FWPC. This study reveals the potential benefits of utilising a potable alcohol derived FWPC on the growth and feed utilisation performance of Nile tilapia, with no apparent adverse impacts on general health and physiological status as determined by gut and liver function and related plasma metabolic status. The results are discussed in the context of a promising protein ingredients source for tropical fish aquaculture.

一种利用酿酒酵母酵母的新型技术先进的发酵小麦面筋和酵母来源蛋白 (FWPC) 副产品对尼罗罗非鱼幼鱼Oreochromis niloticus进行了评估. 半纯化基础日粮（对照处理）的蛋白质成分逐渐被发酵小麦浓缩蛋白 (FWPC) 替代，占日粮总蛋白质的 10%、20% 和 30%，以产生对测试成分的斜率分析响应与“理想蛋白质”必需氨基酸 EAA 平衡来源相比。在为期 8 周的饲喂试验后，饲喂 10% 和 20% FWPC 的罗非鱼表现出与饲喂基于 FM 的对照日粮相似的性能，而饲喂 30% FWPC 的鱼与其他处理相比生长性能最低（P ≤ 0.05）。近似成分分析表明，体蛋白、脂质、灰分、NFE和总能量不受膳食处理的影响（P≥0.05）。血浆丙氨酸氨基转移酶 (ALAT) 和天冬氨酸氨基转移酶 (ASAT) 均作为尼罗罗非鱼肝功能的指标进行测量。在 ALAT 和 ASAT 活动中没有看到任何影响。与其他处理相比，饲喂 30% FWPC 的罗非鱼表现出 HSI 降低 (P ≤ 0.05)。此外，肝脏和肠道组织的组织学比较没有显示健康受损的迹象，但 FWPC 的加入提高了后肠中杯状细胞的数量（P ≤ 0.05）。微绒毛高度和密度的超微结构也显着增加，显示了 FWPC 的功能方面。本研究揭示了利用饮用酒精衍生的 FWPC 对尼罗罗非鱼的生长和饲料利用性能的潜在益处，对肠道和肝功能以及相关血浆代谢状态所确定的一般健康和生理状态没有明显的不利影响。