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High-throughput optical assays for sensing serine hydrolases in living systems and their applications
Trends in Analytical Chemistry ( IF 13.1 ) Pub Date : 2022-04-08 , DOI: 10.1016/j.trac.2022.116620
Qiang Jin , Li-Lin Song , Le-Le Ding , Jing Zhang , Dan-Dan Wang , Yun-Qing Song , Li-Wei Zou , Guang-Bo Ge

Serine hydrolases (SHs) comprise a large family of hydrolytic enzymes that play crucial roles in numerous physiological and pathological processes, such as blood coagulation, cancer, inflammation, and diabetes. This review summarizes the newest advances in the design and development of specific optical substrates for mammalian SHs and related high-throughput optical assays for sensing target SHs in living systems, as well as their applications in bioanalytical and biomedical fields. The design principles and the response modes for SHs optical substrates, as well as the structural information and kinetic parameters of reported probes for mammalian SHs, have been well summarized. Furthermore, the biomedical applications of SHs optical substrates in various fields, including sensing and imaging analysis of target enzyme in biological systems, high-throughput screening of enzyme inhibitors, have been demonstrated. Finally, the challenges and future perspectives in this field are also highlighted.



中文翻译:

检测生命系统中丝氨酸水解酶的高通量光学检测及其应用

丝氨酸水解酶 (SH) 包含一大类水解酶,它们在许多生理和病理过程中发挥关键作用,例如凝血、癌症、炎症和糖尿病。本综述总结了哺乳动物 SHs 特定光学底物设计和开发的最新进展,以及用于传感生命系统中目标 SHs 的相关高通量光学检测方法,以及它们在生物分析和生物医学领域的应用。总结了SHs光学底物的设计原理和响应模式,以及已报道的哺乳动物SHs探针的结构信息和动力学参数。此外,SHs光学底物在各个领域的生物医学应用,包括生物系统中目标酶的传感和成像分析,酶抑制剂的高通量筛选已得到证实。最后,还强调了该领域的挑战和未来前景。

更新日期:2022-04-08
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