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Dynamical Change of Cell Mechanical Properties Accompanying and Modulating Metastasis Process
IEEE Transactions on NanoBioscience ( IF 3.9 ) Pub Date : 2022-04-08 , DOI: 10.1109/tnb.2022.3165871
Na Liu , Xiaoyan Yu , Ziheng Chen , Tao Yue , Yuanyuan Liu , Chen Yang , Haowen Jiang , Huayan Pu

The dynamical change of cellular mechanical properties plays an important role in cell metastasis process, while how the cancer cells modulate their mechanical properties during metastasis are still not fully understood. In this report, the cellular detaching and seeding processes, two vital steps of cell metastasis, were simulated in vitro using a self-developed protocol and characterized by the dynamical mechanical properties using AFM. The measured results show that cells decrease their stiffness and increase their surface adhesion force as they are detaching from substrate, while cells present an opposite change in mechanical properties as they seeding. Additionally, the effect of anti-cancer drug (docetaxel) on the detaching and attaching process of cancer cells (PC-3) was also investigated from the aspect of mechanical properties. The results shows that the docetaxel can increase stiffness, decrease surface adhesion force of PC-3 cell, and slow down the change speed of these mechanical properties during PC-3 cell detaching and seeding process. These discoveries demonstrated that a dynamical change of cell mechanical properties is required for cancer cell metastasis, which provide a new drug development strategy for cancer treatment.

中文翻译:

伴随和调节转移过程的细胞力学特性的动态变化

细胞力学特性的动态变化在细胞转移过程中起着重要作用,而癌细胞在转移过程中如何调节其力学特性仍未完全了解。在本报告中,细胞分离和接种过程是细胞转移的两个重要步骤,使用自行开发的方案在体外模拟,并使用 AFM 以动态机械特性为特征。测量结果表明,细胞在脱离基质时会降低其刚度并增加其表面粘附力,而细胞在接种时会出现相反的机械性能变化。此外,还从力学性能方面研究了抗癌药物(多西紫杉醇)对癌细胞(PC-3)脱离和附着过程的影响。结果表明,多西紫杉醇可增加PC-3细胞的刚度,降低其表面粘附力,减缓PC-3细胞脱离和接种过程中这些力学性能的变化速度。这些发现表明癌细胞转移需要细胞力学特性的动态变化,这为癌症治疗提供了新的药物开发策略。
更新日期:2022-04-08
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