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Role of hemoglobin alpha and hemoglobin beta in non-small-cell lung cancer based on bioinformatics analysis
Molecular Carcinogenesis ( IF 4.6 ) Pub Date : 2022-04-08 , DOI: 10.1002/mc.23404
Ning Kang 1 , Wen-Jia Qiu 2 , Bin Wang 1 , Dong-Fang Tang 1 , Xiao-Yong Shen 1
Affiliation  

The differentially expressed genes (DEGs) were identified and screened differentially in non-small-cell lung cancer (NSCLC) using information from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus databases, and the correlation of DEGs in protein interaction, function, and pathway enrichment were analyzed to search for new biomarkers and potential therapeutic targets for NSCLC. Protein–protein interaction network (PPI) analysis showed that CDK1 and GNGT1 were the most significantly upregulated hub nodes, while FPR2 was the most significantly downregulated. Gene Ontology enrichment analysis showed that upregulated DEGs were significantly enriched in protein heterodimerization activity and other functions, while downregulated DEGs were enriched in functions such as heparin-binding. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that upregulation of DEGs were significantly associated with neuroactive ligand-receptor interaction pathways, while downregulation of DEGs were significantly associated with malaria pathways. According to the analysis results, we identified hemoglobin alpha (HBA) and hemoglobin beta (HBB) as the genes of interest for further study. Through tissue level and cell level experiments, we found that the expressions of HBA and HBB in NSCLC tissues were significantly lower than those in paracancerous tissues, and downregulation of HBA and HBB could significantly affect the proliferation ability of NSCLC cells. In addition, we also found that changes in HBA and HBB may affect NSCLC cells through the p38/MAPK pathway and JNK pathway, and ultimately affect the occurrence and development of NSCLC.

中文翻译:

基于生物信息学分析的血红蛋白α和血红蛋白β在非小细胞肺癌中的作用

使用癌症基因组图谱 (TCGA) 和基因表达综合数据库中的信息以及 DEG 在蛋白质相互作用、功能、分析通路富集以寻找新的生物标志物和潜在的非小细胞肺癌治疗靶点。蛋白质-蛋白质相互作用网络(PPI)分析表明,CDK1 和 GNGT1 是最显着上调的枢纽节点,而 FPR2 是最显着下调的。基因本体富集分析表明,上调的DEGs显着富集蛋白质异二聚化活性和其他功能,而下调的DEGs富集肝素结合等功能。京都基因百科全书和基因组通路分析表明,DEGs 的上调与神经活性配体-受体相互作用通路显着相关,而 DEGs 的下调与疟疾通路显着相关。根据分析结果,我们确定了血红蛋白α(HBA)和血红蛋白β(HBB)作为进一步研究的感兴趣基因。通过组织水平和细胞水平实验,我们发现HBA和HBB在NSCLC组织中的表达明显低于癌旁组织,HBA和HBB的下调可显着影响NSCLC细胞的增殖能力。此外,我们还发现HBA和HBB的变化可能通过p38/MAPK通路和JNK通路影响NSCLC细胞,
更新日期:2022-04-08
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