Atrazine (ATZ) is one of the pesticides mostly widely used in Brazil; several studies have shown the toxic effects of this herbicide on aquatic organisms such as fish. Thus, it is absolutely necessary finding alternatives to protect the health of fish, mainly of species commercially important for aquaculture, which may be exposed to atrazine deriving from agricultural runoff. The aim of the current study was to investigate interactions between dietary supplementation with vitamin C (Vit C) antioxidant and exposure to ATZ in Rhamdia quelen fish exposed to this herbicide. R. quelen specimens were divided into four groups: (1) CTRL, (2) VitC, (3) ATZ, (4) ATZ + VitC. Groups 3 and 4 were exposed to ATZ (10 µg L⁻¹) for 96 h, after 30 days of VitC supplementation (1 g kg⁻¹). Liver samples were collected for biomarker assays. Group 4 was the only group presenting decreased protein carbonyl content. Non-protein thiol (NPSH) levels were significantly higher in groups VitC, ATZ and ATZ + VitC than in CTRL. Group ATZ + VitC presented significant increase in glutatione-peroxidase (GPx) activity in comparison to the other investigated groups. Ascorbic acid (AA) levels were significantly higher in group VitC and lower in group ATZ. Therefore, interactions between herbicide ATZ and dietary supplementation with Vit C have shown biochemical changes in R. quelen fish. Thus, dietary supplements with adequate amounts of Vit C can be added as antioxidants to the diet of fish bred in aquaculture systems in order to protect them from exposure to ATZ.

阿特拉津 (ATZ) 是巴西最广泛使用的农药之一；几项研究表明，这种除草剂对鱼类等水生生物具有毒性作用。因此，绝对有必要寻找替代方法来保护鱼类的健康，主要是对水产养殖具有商业重要性的物种，这些鱼类可能会接触到来自农业径流的阿特拉津。本研究的目的是研究膳食补充剂与维生素 C (Vit C) 抗氧化剂与暴露于这种除草剂的Rhamdia quelen鱼接触 ATZ 之间的相互作用。R. quelen标本分为四组：（1）CTRL，（2）VitC，（3）ATZ，（4）ATZ + VitC。第 3 组和第 4 组暴露于 ATZ (10 µg L ^-1) 96 小时，在补充 VitC 30 天后 (1 g kg ^-1 )。收集肝脏样本用于生物标志物测定。第 4 组是唯一呈现降低蛋白质羰基含量的组。VitC、ATZ 和 ATZ + VitC 组的非蛋白硫醇 (NPSH) 水平显着高于 CTRL。与其他研究组相比，ATZ + VitC 组的谷氨酸过氧化物酶 (GPx) 活性显着增加。VitC 组的抗坏血酸 (AA) 水平显着升高，而 ATZ 组的抗坏血酸 (AA) 水平显着降低。因此，除草剂 ATZ 和添加维生素 C 的膳食补充剂之间的相互作用已显示出R. quelen的生化变化鱼。因此，可以将含有足量维生素 C 的膳食补充剂作为抗氧化剂添加到水产养殖系统中养殖的鱼的饮食中，以保护它们免受 ATZ 的影响。