当前位置: X-MOL 学术The Journal of Cell Biology › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
DAPLE orchestrates apical actomyosin assembly from junctional polarity complexes
The Journal of Cell Biology Pub Date : 2022-04-07 , DOI: 10.1083/jcb.202111002
Arthur Marivin 1 , Rachel Xi-Yeen Ho 1 , Mikel Garcia-Marcos 1
Affiliation  

Establishment of apicobasal polarity and the organization of the cytoskeleton must operate coordinately to ensure proper epithelial cell shape and function. However, the precise molecular mechanisms by which polarity complexes directly instruct the cytoskeletal machinery to determine cell shape are poorly understood. Here, we define a mechanism by which the PAR polarity complex (PAR3–PAR6–aPKC) at apical cell junctions leads to efficient assembly of the apical actomyosin network to maintain epithelial cell morphology. We found that the PAR polarity complex recruits the protein DAPLE to apical cell junctions, which in turn triggers a two-pronged mechanism that converges upon assembly of apical actomyosin. More specifically, DAPLE directly recruits the actin-stabilizing protein CD2AP to apical junctions and, concomitantly, activates heterotrimeric G protein signaling in a GPCR-independent manner to favor RhoA-myosin activation. These observations establish DAPLE as a direct molecular link between junctional polarity complexes and the formation of apical cytoskeletal assemblies that support epithelial cell shape.

中文翻译:

DAPLE 协调连接极性复合物的顶端肌动球蛋白组装

顶端基底极性的建立和细胞骨架的组织必须协调运作,以确保上皮细胞适当的形状和功能。然而,人们对极性复合物直接指导细胞骨架机制确定细胞形状的精确分子机制知之甚少。在这里,我们定义了一种机制,通过该机制,顶端细胞连接处的 PAR 极性复合物(PAR3-PAR6-aPKC)导致顶端肌动球蛋白网络的有效组装,以维持上皮细胞形态。我们发现 PAR 极性复合物将蛋白质 DAPLE 募集到顶端细胞连接处,这反过来又触发了汇聚顶端肌动球蛋白组装的双管齐下的机制。更具体地说,DAPLE 直接将肌动蛋白稳定蛋白 CD2AP 募集到顶端连接处,同时以不依赖 GPCR 的方式激活异三聚体 G 蛋白信号传导,以促进 RhoA-肌球蛋白激活。这些观察结果表明,DAPLE 是连接极性复合物与支持上皮细胞形状的顶端细胞骨架组件形成之间的直接分子联系。
更新日期:2022-04-07
down
wechat
bug