The stay-green trait is of considerable importance in extending the shelf life of green pepper fruit (Capsicum annuum L.) and in enhancing the appearance of ornamental plants. The study revealed the genetic and regulatory mechanisms of the stay-green trait in pepper, which will aid in the selection of ornamental pepper varieties. In this study, a pepper mutant with stay-green fruit named TNX348 was identified from a germplasm resource bank. Two segregating populations were constructed using the stay-green mutant TNX348 and then used in bulked segregant analysis combined with RNA sequencing and linkage analyses. The causal gene of the stay-green trait was mapped to an approximately 131-kb region, and a senescence-induced chloroplast protein gene, CaSGR1 (Capana01g000359), was identified as a candidate gene. Sequencing analysis revealed a G→A single-base mutation of CaSGR1 in TNX348 that led to early termination of translation. Based on the single-base mutation, a single nucleotide polymorphism (SNP) marker co-segregating with the stay-green trait was developed. Furthermore, in transcriptome analysis, expression patterns of 11 hormone transduction-related transcription factors, such as abscisic acid-insensitive (ABI), abscisic acid-responsive element-binding factor (ABF), and NAC transcription factor, were similar or opposite to that of CaSGR1. The results indicated that the transcription factors might mediate chlorophyll degradation by regulating the expression of CaSGR1.

保持绿色性状对于延长青椒果实 ( Capsicum annuum L.)的保质期和改善观赏植物的外观具有相当重要的意义。该研究揭示了辣椒留绿性状的遗传调控机制，有助于观赏辣椒品种的选育。在本研究中，从种质资源库中鉴定出一个果实常绿的辣椒突变体 TNX348。使用保持绿色的突变体 TNX348 构建了两个分离种群，然后用于结合 RNA 测序和连锁分析的批量分离分析。留绿性状的致病基因被定位到一个大约 131-kb 的区域，以及一个衰老诱导的叶绿体蛋白基因CaSGR1 ( Capana01g000359), 被确定为候选基因。测序分析显示 TNX348 中CaSGR1的 G→A 单碱基突变导致翻译提前终止。基于单碱基突变，开发了与留绿性状共分离的单核苷酸多态性（SNP）标记。此外，在转录组分析中，脱落酸不敏感（ABI）、脱落酸反应元件结合因子（ABF）和NAC转录因子等11种激素转导相关转录因子的表达模式与其相似或相反。CaSGR1的。结果表明转录因子可能通过调控CaSGR1的表达介导叶绿素降解。