ABSTRACT

At present, most commercial kits for nucleic acid isolation are silica-based. In this regard, new strategies are under development with the aim of optimizing nucleic acid purification silica-based protocols. This review aims to summarize the evolution of such strategies and highlight the current necessities to be solved by new purification methods based on silica materials. In addition, it will perform a survey of the purified DNA/RNA quality and quantity for a subsequent technique. This information is of vital importance to consider for the success of molecular diagnostics. The text was designed to guide scientists and engineers in choosing suitable DNA/RNA purification techniques for research or bioprocessing applications.

摘要

目前，大多数用于核酸分离的商业试剂盒都是基于硅胶的。在这方面，正在开发新的策略，旨在优化基于硅胶的核酸纯化方案。本综述旨在总结此类策略的演变，并强调基于二氧化硅材料的新纯化方法当前需要解决的问题。此外，还将对纯化后的 DNA/RNA 质量和数量进行调查，以用于后续技术。这些信息对于分子诊断的成功至关重要。该文本旨在指导科学家和工程师为研究或生物加工应用选择合适的 DNA/RNA 纯化技术。