Seamless modification of bacterial chromosomes is widely performed in both theoretical and practical research. For this purpose, excellent counter-selection marker genes with high stringency are needed.

中文翻译：

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Lysis基因E作为高严格性反选择标记的开发和优化

细菌染色体的无缝修饰在理论和实践研究中都得到了广泛的应用。为此，需要具有高度严格性的优秀反选择标记基因。