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Genetic Characterization and Conservation of Grapevine Germplasms in Kayseri Province of Turkey
Erwerbs-Obstbau ( IF 1.3 ) Pub Date : 2022-03-29 , DOI: 10.1007/s10341-022-00665-y
Gülşen Yılmaz , Aydın Uzun , Ali Sabır , Hasan Pınar

Turkey is within the region where the viticulture has been traditionally practiced since ancient times. The Cappadocia location, including the province of Kayseri, is an important grape production area with its autochthonous grape cultivars and unique tradition in history. Due to viticulture history, this region is rich in vine genetic resources. This survey study was carried out in vineyards areas and residential gardens in 23 different regions of Kayseri province between 2017 and 2019. A total of 174 local genotypes from different locations were determined and were further examined through molecular characterization with SSR markers. A total of 112 bands were amplified by 12 SSR primers, all of which were polymorphic with a 100% polymorphism. The highest number of polymorphic bands (18) was produced by the primer Scu8vv, while the lowest number of polymorphic bands (3) was found in VMC8D3 and VMC8E6. An UPGMA dendrogram was created via scoring the bands and the genetic similarity between the genotypes was determined between 0.63–1.0. In conclusion, a wide range of genetic diversity was determined in grapes of Kayseri indicating an ancient residential germplasm collection that could be used for breeding studies.



中文翻译:

土耳其开塞利省葡萄种质的遗传特征和保护

土耳其位于自古以来就以传统方式进行葡萄栽培的地区。卡帕多西亚地区,包括开塞利省,是一个重要的葡萄产区,拥有本土葡萄品种和独特的历史传统。由于葡萄栽培历史,该地区拥有丰富的葡萄遗传资源。这项调查研究于 2017 年至 2019 年间在开塞利省 23 个不同地区的葡萄园区和住宅花园中进行。共确定了来自不同地点的 174 个当地基因型,并通过 SSR 标记的分子表征进一步检查。12条SSR引物共扩增出112条带,均为多态性,多态性为100%。引物 Scu8vv 产生的多态性条带数量最多(18 个),而在 VMC8D3 和 VMC8E6 中发现了最少数量的多态带 (3)。通过对条带评分创建 UPGMA 树状图,确定基因型之间的遗传相似性在 0.63-1.0 之间。总之,在 Kayseri 的葡萄中确定了广泛的遗传多样性,表明可用于育种研究的古老住宅种质收集。

更新日期:2022-03-29
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