Brown planthopper (BPH) has become the most devastating insect pests of rice and a serious threat to rice production. To combat newly occurring virulent BPH populations, it is still urgent to explore more new broad-spectrum BPH resistance genes and integrate them into rice cultivars. In the present study, we explored the genetic basis of BPH resistance in IRGC 8678. We identified and mapped a new resistance gene Bph43 to a region of ~380 kb on chromosome 11. Genes encoding nucleotide-binding domain leucine-rich repeat-containing (NBS-LRR)-type disease resistance proteins or Leucine Rich Repeat family proteins annotated in this region were predicted as the possible candidates for Bph43. Meanwhile, we developed near isogenic lines of Bph43 (NIL-Bph43-9311) in an elite restorer line 9311 background using marker-assisted selection (MAS). The further characterization of NIL-Bph43-9311 demonstrated that Bph43 confers strong antibiosis and antixenosis effects on BPH. Comparative transcriptome analysis revealed that genes related to the defense response and resistance gene-dependent signaling pathway were significantly and uniquely enriched in BPH-infested NIL-Bph43-9311. Our work demonstrated that Bph43 can be deployed as a valuable donor in BPH resistance breeding programs.

中文翻译：

---

水稻（Oryza sativa L.）抗褐飞虱新基因Bph43的分子定位

褐飞虱(BPH)已成为对水稻最具破坏性的害虫，对水稻生产构成严重威胁。为了对抗新出现的恶性BPH种群，探索更多新的广谱BPH抗性基因并将其整合到水稻品种中仍然是当务之急。在本研究中，我们探索了 IRGC 8678 中 BPH 抗性的遗传基础。我们鉴定了一个新的抗性基因Bph43 并将其定位到 11 号染色体上约 380 kb 的区域。 NBS-LRR) 型抗病蛋白或在该区域注释的富含亮氨酸的重复家族蛋白被预测为Bph43的可能候选者。同时，我们在Bph43（NIL-Bph43 -9311) 在精英恢复系 9311 背景中使用标记辅助选择 (MAS)。NIL -Bph43 -9311的进一步表征表明Bph43对 BPH 具有很强的抗菌和抗异种作用。比较转录组分析显示，与防御反应和抗性基因依赖性信号通路相关的基因在 BPH 感染的 NIL-Bph43 -9311 中显着且独特地富集。我们的工作表明Bph43可以作为有价值的供体用于 BPH 抗性育种计划。