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Identification of snow mold tolerance QTL in a landrace winter wheat using linkage mapping
Crop Science ( IF 2.3 ) Pub Date : 2022-03-26 , DOI: 10.1002/csc2.20745
Savannah N. Phipps 1 , Adrienne B. Burke 1 , Kerry Balow 1 , Jared Smith 2 , Timothy Murray 3 , Arron H. Carter 1
Affiliation  

In areas of the Pacific Northwest (PNW), snow mold, a fungal disease with multiple causal agents, can have severe impacts on winter wheat (Triticum aestivum L. subsp. aestivum) yields. Growing tolerant varieties is the best option to manage the disease; however, genetic diversity for the trait is limited in local varieties. The winter wheat landrace PI 173438 has been used in Japan to improve snow mold tolerance but has not been used in PNW breeding programs. A doubled haploid population from a cross between PI 173438 and susceptible variety WA 8137 was used to identify novel quantitative trait loci (QTL) associated with snow mold tolerance that would be validated using marker-assisted selection (MAS) in two separate populations. A linkage map was developed using genotypic data collected on the PI 173438/WA 8137 population, which was then used with phenotypic data in composite interval mapping for QTL analysis. Six QTL were found associated with snow mold tolerance traits, five of which originated from PI 173438. A QTL was found on chromosome 1D that has not been reported before. The remaining QTL occurred on chromosomes that were previously reported to have snow mold tolerant QTL, some of which may be identical. Several of these QTL and linked markers were suitable for testing via MAS on separate populations revealing no significant difference amongst the three or four QTL that could be tested. Such results continue to indicate the complexity of breeding for snow mold tolerance using molecular markers.

中文翻译:

利用连锁作图鉴定地方品种冬小麦抗雪霉性QTL

在太平洋西北部 (PNW) 地区,雪霉病是一种具有多种致病因素的真菌病害,可对冬小麦 ( Triticum aestivum L. subsp. aestivum) 产量。种植耐受性品种是控制疾病的最佳选择;然而,该性状的遗传多样性在当地品种中是有限的。冬小麦地方品种 PI 173438 已在日本用于提高对雪霉病的耐受性,但尚未用于 PNW 育种计划。来自 PI 173438 和易感品种 WA 8137 的杂交的双倍单倍体群体被用于鉴定与雪霉病耐受性相关的新数量性状基因座 (QTL),该基因座将使用标记辅助选择 (MAS) 在两个单独的群体中进行验证。使用在 PI 173438/WA 8137 群体上收集的基因型数据开发了连锁图谱,然后将其与表型数据一起用于 QTL 分析的复合区间作图。发现 6 个 QTL 与耐雪霉性状相关,其中 5 个源自 PI 173438。在 1D 染色体上发现了一个以前没有报道过的 QTL。剩余的 QTL 发生在先前报道的具有耐雪霉 QTL 的染色体上,其中一些可能是相同的。这些 QTL 和连锁标记中的一些适合通过 MAS 对单独的群体进行测试,表明可以测试的三个或四个 QTL 之间没有显着差异。这样的结果继续表明使用分子标记培育耐雪霉病的复杂性。这些 QTL 和连锁标记中的一些适合通过 MAS 对单独的群体进行测试,表明可以测试的三个或四个 QTL 之间没有显着差异。这样的结果继续表明使用分子标记培育耐雪霉病的复杂性。这些 QTL 和连锁标记中的一些适合通过 MAS 对单独的群体进行测试,表明可以测试的三个或四个 QTL 之间没有显着差异。这样的结果继续表明使用分子标记培育耐雪霉病的复杂性。
更新日期:2022-03-26
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