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Protective Effect of Food Against Inactivation of Human Coronavirus OC43 by Gastrointestinal Fluids
Food and Environmental Virology ( IF 3.4 ) Pub Date : 2022-03-23 , DOI: 10.1007/s12560-022-09520-5
Jennifer Harlow 1 , Matthew Dallner 1 , Neda Nasheri 1, 2
Affiliation  

The involvement of the gastrointestinal (GI) tract in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection has been reported in multiple studies. Since it has been demonstrated that human intestinal epithelial cells support productive viral replication and that a substantial portion of infected individuals shed the virus in feces, the possibility of fecal–oral and fecal-respiratory modes of transmission have been proposed for SARS-CoV-2. In order to establish viral replication in the intestine, enteric viruses need to retain their infectivity in often low pH gastric fluids, and in intestinal fluids, which contain digestive enzymes and bile salts. In this study, we examined whether human coronaviruses OC43 (HCoV-OC43) can remain infectious in simulated GI fluids that models human fasting-state and fed-state, in the presence or absence of food. We demonstrated that except for fasting-state gastric fluid (pH 1.6), the virus can remain infectious in all other gastrointestinal fluids for 1 h. Furthermore, we demonstrated that presence of food could significantly improve viral survival in gastric fluids. Therefore, this study provides evidence that ingestion with food could protect the virus against inactivation by the GI fluids.



中文翻译:

食物对胃肠液对人冠状病毒OC43灭活的保护作用

多项研究报告了胃肠道 (GI) 参与严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 感染。由于已经证明人类肠道上皮细胞支持有效的病毒复制,并且很大一部分感染者在粪便中排出病毒,因此已经提出 SARS-CoV-2 可能存在粪-口和粪-呼吸道传播方式. 为了在肠道中建立病毒复制,肠道病毒需要在通常是低 pH 值的胃液和含有消化酶和胆汁盐的肠道液中保持其传染性。在这项研究中,我们检查了人类冠状病毒 OC43 (HCoV-OC43) 是否可以在模拟人类禁食状态和进食状态的模拟胃肠液中保持传染性,在有或没有食物的情况下。我们证明,除了空腹状态胃液(pH 1.6)外,病毒可以在所有其他胃肠液中保持传染性 1 小时。此外,我们证明了食物的存在可以显着提高胃液中的病毒存活率。因此,这项研究提供的证据表明,与食物一起摄入可以保护病毒免受胃肠液的灭活。

更新日期:2022-03-23
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