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Calorie restriction changes the anxiety-like behaviour of ageing male Wistar rats in an onset- and duration-dependent manner
Mechanisms of Ageing and Development ( IF 5.3 ) Pub Date : 2022-03-21 , DOI: 10.1016/j.mad.2022.111666
Milica Prvulovic 1 , Smilja Todorovic 1 , Desanka Milanovic 1 , Milena Jovic 1 , Predrag Vujovic 2 , Selma Kanazir 1 , Aleksandra Mladenovic 1
Affiliation  

Although initially recognized as a universally beneficial approach for the prevention of age-related impairments, the outcome of calorie restriction (CR) is now known to depend on several factors, most notably the age of the subject at the CR commencement, and CR duration. We aimed to examine if and how CR affects anxiety-like behaviour when it is introduced at middle age and late middle age. In addition, as the dopaminergic system is one of the main neurotransmitter systems involved in controlling anxiety, we examined the expression of dopamine receptors (D1R, D2R) in the cortex, striatum, and mesencephalon of male Wistar rats of varying ages. The study was performed on rats fed ad libitum (AL) or exposed to calorie restriction (60% of AL intake). Open field and light-dark tests were used to study anxiety-like behaviour, while PCR and Western blot were used to examine the expression of dopamine receptors. Calorie restriction implemented at middle-age led to variable outcomes on anxiety-like behaviour, while CR implemented at late middle age increased anxiety and decreased the availability of D2R levels in the cortex and mesencephalon. Taken together, these results advise caution when implementing calorie restriction late in life.



中文翻译:

热量限制以发作和持续时间依赖性方式改变衰老雄性 Wistar 大鼠的焦虑样行为

虽然最初被认为是预防与年龄相关的损伤的普遍有益的方法,但现在已知卡路里限制 (CR) 的结果取决于几个因素,最显着的是受试者在 CR 开始时的年龄和 CR 持续时间。我们旨在研究在中年和中年后期引入 CR 是否以及如何影响焦虑样行为。此外,由于多巴胺能系统是参与控制焦虑的主要神经递质系统之一,我们检测了多巴胺受体(D 1 R,D 2R) 在不同年龄的雄性 Wistar 大鼠的皮层、纹状体和中脑。该研究是对随意喂食 (AL) 或受到热量限制(AL 摄入量的 60%)的大鼠进行的。开放视野和明暗测试用于研究焦虑样行为,而 PCR 和蛋白质印迹用于检查多巴胺受体的表达。中年实施的卡路里限制导致焦虑样行为的不同结果,而中年后期实施的 CR 增加了焦虑并降低了皮层和中脑D 2 R 水平的可用性。综上所述,这些结果表明在晚年实施卡路里限制时要谨慎。

更新日期:2022-03-21
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