Uncontrolled hemorrhage of deep, narrow and non-compressible perforating wounds is responsible for many trauma deaths. In this work, a novel biodegradable hemostatic sponge based on thermosensitive carboxymethyl chitin was prepared via simple cryo-regeneration process without using any crosslinkers. The collagen and polydopamine were added to further enhance mechanical and hemostatic properties of the sponge. All the carboxymethyl chitin based sponges showed high strength with excellent water/blood-triggered shape memory property, and the highest compressive fracture wet-strength could reach about 291.2 kPa, which was almost higher than those of many reported biodegradable hemostatic sponges pre-swelled in water. More importantly, the carboxymethyl chitin-collagen-polydopamine sponges displayed much better blood-clotting capacity and superior hemostasis performance than gauze and clinically used collagen sponge iRegene^@ in vitro and in the rat liver perforating wound model. This study revealed a facile strategy to construct the effective carboxymethyl chitin based hemostatic sponges for the deep and non-compressible perforating wound.

深部、狭窄和不可压缩的穿孔伤口不受控制的出血是造成许多创伤死亡的原因。在这项工作中，通过简单的低温再生工艺，不使用任何交联剂，制备了一种基于热敏羧甲基甲壳素的新型可生物降解止血海绵。添加胶原蛋白和聚多巴胺以进一步增强海绵的机械和止血性能。所有羧甲基甲壳素基海绵均表现出高强度，具有优异的水/血触发形状记忆性能，最高压缩断裂湿强度可达约291.2 kPa，几乎高于许多报道的预溶胀的可生物降解止血海绵。水。更重要的是，^@在体外和大鼠肝脏穿孔伤口模型中。这项研究揭示了一种简便的策略来构建有效的羧甲基甲壳素基止血海绵，用于深部和不可压缩的穿孔伤口。