Nezara viridula (L.) (Hemiptera: Pentatomidae), commonly known in the U.S. as the southern green stink bug (SGSB), is a cosmopolitan, highly polyphagous feeder that causes severe damage to a wide range of agronomically important crops such as fruit, vegetable, grain, tobacco, and cotton, throughout much of the United States, and is a global pest of considerable ecological, agricultural, and economical interest. During dissection of female Nz. viridula, conspicuous black and brown spots or lesions were observed on various internal organs. To determine the cause of these spots or lesions, tissues of fat body, spermatheca, ovaries, and ovulated eggs were collected from healthy and infected individuals. The gross morphology of the spots was characterized, and the microorganisms associated with the infection were identified by amplicon sequencing of the V4 region of the small subunit rRNA gene. The presence of a microsporidian pathogen Nosema maddoxi, Becnel, Solter, Hajek, Huang, Sanscrainte, & Estep (Microsporidia: Nosematidae) which has been observed on other species of stink bug, was evidenced for the first time. The characterization of the gross morphology of this associated microsporidian may enable more rapid determination of microsporidia infection in stink bug colonies and field populations.

中文翻译：

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Nezara viridula（半翅目：Pentatomidae）患病组织的大体形态学和相关微孢子虫的分子特征。

Nezara viridula (L.) (Hemiptera: Pentatomidae)，在美国通常被称为南方绿蝽 (SGSB)，是一种世界性的、高度杂食性的饲养者，对水果等多种重要农作物造成严重损害，蔬菜、谷物、烟草和棉花，遍及美国大部分地区，是一种具有相当大的生态、农业和经济利益的全球害虫。在解剖女性 Nz。各种内脏器官可见绿色、明显的黑褐色斑点或病变。为了确定这些斑点或病变的原因，从健康和受感染的个体中收集了脂肪体、受精囊、卵巢和排卵的组织。对斑点的大体形态进行了表征，并通过小亚基rRNA基因V4区的扩增子测序鉴定出与感染相关的微生物。首次证实了在其他种类的臭虫上观察到的微孢子虫病原体 Nosema maddoxi、Becnel、Solter、Hajek、Huang、Sanscrainte 和 Estep（微孢子虫：Nosematidae）的存在。这种相关微孢子虫的大体形态特征可以更快速地确定臭虫菌落和田间种群中的微孢子虫感染。