当前位置: X-MOL 学术Eur. J. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Differential responses of pear cultivars to Erwinia amylovora infection; evidences of involvement the hypersensitivity response in pear resistance to fire blight
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2022-01-30 , DOI: 10.1007/s10658-021-02448-9
Javad Erfani-Moghadam , Abdolkarim Zarei

Fire blight, a disease caused by the bacterium Erwinia amylovora, is a serious threat for pear production worldwide. In order to shed light on the mechanisms underlying fire blight resistance, a comparable study was conducted using ‘Dargazi’ and ‘Duchess’, two pear cultivars displaying contrasting responses to fire blight. The activity of ascorbate peroxidase (APX), catalase (CAT), and guaiacol peroxidase (POD) as well as phenylalanine ammonia-lyase (PAL), the enzyme associated with phenolic metabolism, and the relevant biochemical compounds including total phenol and flavonoid contents were compared in two cultivars in response to E. amylovora. All biochemical parameters were affected by pathogen attack. However, the enzymatic responses of resistant and susceptible pear cultivars were completely different following E. amylovora infection. In ‘Dargazi’ the activity of CAT and APX were significantly suppressed in response to the pathogen attack during the early days after bacterial inoculation, while they were significantly activated in 'Duchess' at the same stages. The POD showed a different pattern of activity from two other antioxidant enzymes. Pathogen attack induced the phenylpropanoid metabolism and caused an increase in PAL activity as well as total phenol and flavonoid content in the two pear cultivars, but a significantly higher rise was recorded in 'Dargazi' than in 'Duchess'. Our data strongly suggest that the hypersensitivity response (HR) may contribute to the high resistance of 'Dargazi' to E. amylovora. Results of present study provide valuable information about the mechanisms underlying the fire blight resistance in 'Dargazi' cultivar which would be highly beneficial for engineering fire blight resistance Pyrus cultivars.



中文翻译:

梨品种对解淀粉欧文氏菌感染的差异反应;参与梨对火疫病抗性的超敏反应的证据

火疫病是一种由解酸欧文氏菌引起的疾病,对全世界的梨生产构成严重威胁。为了阐明抗火疫病的潜在机制,使用“Dargazi”和“Duchess”进行了一项类似研究,这两种梨品种对火疫病的反应不同。抗坏血酸过氧化物酶 (APX)、过氧化氢酶 (CAT) 和愈创木酚过氧化物酶 (POD) 以及与酚类代谢相关的酶苯丙氨酸解氨酶 (PAL) 的活性以及包括总酚和类黄酮含量在内的相关生化化合物的活性均在针对E. amylovora在两个品种中进行比较. 所有生化参数都受到病原体攻击的影响。然而,抗梨品种和易感梨品种的酶促反应在E. amylovora之后完全不同感染。在“Dargazi”中,CAT 和 APX 的活性在细菌接种后的早期对病原体攻击的反应被显着抑制,而在“Duchess”中它们在相同阶段被显着激活。POD 显示出与其他两种抗氧化酶不同的活性模式。病原体攻击诱导了苯丙素代谢并导致两个梨品种中 PAL 活性以及总酚和类黄酮含量的增加,但在 'Dargazi' 中记录到的上升显着高于在 'Duchess' 中。我们的数据强烈表明,超敏反应 (HR) 可能有助于 'Dargazi' 对E. amylovora的高抗性. 本研究的结果提供了有关'Dargazi'品种抗火疫病机制的宝贵信息,这将非常有利于工程抗火疫病品种。

更新日期:2022-01-30
down
wechat
bug