Citrus black spot (CBS), caused by Phyllosticta citricarpa (McAlpine) van der Aa, is a disease that affects Citrus spp. causing production losses, high costs associated with management, and constraints on the fresh market trade. The present investigation aimed to confirm the identity of the CBS pathogen as P. citricarpa and determine its mating-type distribution in Cuba. Phyllosticta isolates were collected from major Cuban citrus-producing provinces (Matanzas, Cienfuegos, Camagüey, and Santiago de Cuba). P. citricarpa and the endophyte P. capitalensis (Henn.) were identified by polyphasic taxonomy, integrating morpho-physiological characteristics with the phylogenetic analysis of ITS, actA, LSU and tef1 loci. Mating-type (MAT) analysis revealed that only the MAT1-2 idiomorph of P. citricarpa is present in Cuba, providing evidence of an atypical CBS disease cycle. These results contribute to a better understanding of the epidemiology of CBS in Cuba and the improvement of CBS management.

柑橘黑斑病 (CBS) 由柑橘叶霉(McAlpine) van der Aa 引起，是一种影响柑橘属的疾病。造成生产损失、与管理相关的高成本以及对新鲜市场贸易的限制。本调查旨在确认 CBS 病原体的身份为P. citricarpa并确定其在古巴的交配型分布。从古巴主要的柑橘生产省（马坦萨斯、西恩富戈斯、卡马圭和古巴圣地亚哥）收集叶霉分离物。P. citricarpa和内生菌P. capitalensis(Henn.) 通过多相分类法鉴定，将形态生理特征与 ITS、actA、LSU 和tef1基因座的系统发育分析相结合。交配型 ( MAT)分析显示，古巴仅存在柠檬果的MAT1-2异形，为非典型 CBS 疾病周期提供了证据。这些结果有助于更好地了解古巴 CBS 的流行病学和改进 CBS 管理。