Archives of Animal Nutrition ( IF 2 ) Pub Date : 2022-03-15 , DOI: 10.1080/1745039x.2021.2003682 Alessia Trimigno 1 , Bekzod Khakimov 1 , Michelle Lauge Quaade 2 , Oliver Legarth Honoré 2 , Tove Clausen 3 , Karoline Blaabjerg 3 , Søren Balling Engelsen 1 , Anne Sofie Vedsted Hammer 2
ABSTRACT
The introduction of high amounts of cooked poultry offal in mink feed has been associated with health problems in growing mink. Cooking mink feed is a convenient way of reducing microbiological activity, but it may have a negative effect on raw material quality and animal welfare. This study investigates growth and health of mink fed raw or cooked poultry offal and describes urinary and blood plasma metabolic changes related to the feeding. A total of 65 male mink were divided in three feeding groups, two fed cooked offal and one group fed raw offal, and the plasma and urine samples were collected at 3 time points during the growth. Both bio-fluids and feed samples were measured by 1H NMR spectroscopy and resulted metabolomics data were analysed using univariate and multivariate statistical methods that revealed dominating effect of the mink growth stages and to a less extent the feeding regime. Metabolome differences in relation to low body mass index (BMI) and kidney lesions were observed in plasma. Disease and decrease in BMI was associated with high creatinine and dimethylglycine content in plasma. These molecules were also particularly indicative of the cooked feeds. Moreover, low urinary taurine levels were also associated with disease and low BMI. Individual mink appeared to show negative effects of the cooked feed diet, including impaired growth and gross pathological lesions involving the kidneys. This may be related to the absorption of essential metabolites such as amino acids and fats, necessary for mink growth, that are negatively impacted by the cooking process.
中文翻译:
生或熟家禽内脏干预后农场水貂(Neovison vison)的尿液和血浆代谢组:1H NMR 研究
摘要
在水貂饲料中加入大量煮熟的家禽内脏与水貂生长过程中的健康问题有关。烹调水貂饲料是降低微生物活动的便捷方式,但可能对原料质量和动物福利产生负面影响。本研究调查了喂食生的或煮熟的家禽内脏的貂的生长和健康状况,并描述了与喂食相关的尿液和血浆代谢变化。将65只雄性水貂分为3个饲喂组,2组饲喂熟内脏,1组饲喂生内脏,在生长过程中的3个时间点采集血浆和尿液样本。生物流体和饲料样品均由1使用单变量和多变量统计方法分析 H 核磁共振波谱和所得代谢组学数据,这些方法揭示了水貂生长阶段的主导效应,以及在较小程度上的饲养方式。在血浆中观察到与低体重指数 (BMI) 和肾脏病变相关的代谢组差异。疾病和 BMI 下降与血浆中高肌酐和二甲基甘氨酸含量有关。这些分子也特别指示煮熟的饲料。此外,低尿牛磺酸水平也与疾病和低 BMI 相关。个体水貂似乎表现出熟食饮食的负面影响,包括生长受损和涉及肾脏的严重病理损伤。这可能与水貂生长所必需的氨基酸和脂肪等必需代谢物的吸收有关,