ABSTRACT

This paper presents a laboratory study using terrestrial gastropods. Two different life stages of a cosmopolitan terrestrial gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum) were used. The objective of this study was to evaluate the effect of cadmium on a target organism (H. aspersa aspersa) and the possible transfer of cadmium to the snail tissues. The sensitivity of different snails’ life stages exposed to different levels of cadmium was evaluated. The ecotoxicity tests for juvenile snails were performed according to ISO 15952:2018 standard and lasted for 28 days. During that period, the growth inhibition (both in mass and shell diameter) was observed. The embryotoxicity experiments lasted for 14 days and the hatching success was evaluated. The IC₅₀ (inhibition concentration) values for juveniles and eggs were calculated as 68.2 and 7.6 mg/L of Cd²⁺, respectively. All individuals were frozen for the determination of cadmium content by atomic absorption spectrometry (AAS). The results proved the increased levels of cadmium in the tissues while the mass of the snails decreased. Our results confirm that snail H. aspersa aspersa (and its life stages) is a very suitable laboratory organism for soil ecotoxicity assessment.

摘要

本文介绍了一项使用陆地腹足动物的实验室研究。使用了世界性陆地腹足动物Helix aspersa aspersa（同义词Cantareus aspersus或Cornu aspersum）的两个不同生命阶段。本研究的目的是评估镉对目标生物体 ( H. aspersa aspersa ) 的影响) 和镉可能转移到蜗牛组织。对不同生命阶段的蜗牛暴露于不同镉水平的敏感性进行了评估。幼螺生态毒性试验按照 ISO 15952:2018 标准进行，试验时间为 28 天。在此期间，观察到生长抑制（质量和壳直径）。胚胎毒性实验持续了14天，并评估了孵化成功率。幼鱼和鸡蛋的 IC ₅₀（抑制浓度）值计算为 68.2 和 7.6 mg/L Cd ²⁺， 分别。将所有个体冷冻以通过原子吸收光谱法（AAS）测定镉含量。结果证明组织中镉的含量增加，而蜗牛的质量却减少了。我们的研究结果证实，蜗牛H. aspersa aspersa（及其生命阶段）是一种非常适合用于土壤生态毒性评估的实验室生物。