In an effort to impart antimicrobial behavior to cotton using renewable and environmentally benign components, fabric was treated using layer-by-layer (LbL) assembly. Alternating layers of phytic acid (PA) and chitosan (CH) were deposited from water. Cotton coated with four and eight bilayers (BLs) of anionic PA and cationic CH, with and without adding copper (II) sulfate (CuSO₄) into the CH solution, was evaluated in accordance with the American Association of Textile Chemists and Colorists (AATCC) TM 100-2019 test method. Gram-positive Staphylococcus aureus and gram-negative Klebsiella pneumoniae were used to determine whether the addition of copper salt into a CH network improves antibacterial efficacy. The copper ions were successfully incorporated into the CH network formed using LbL assembly onto cotton fibers. The location of deposited copper ions was determined with electron microscopy. Just four BLs of PA/CH–copper (II) sulfate kill 100% of gram-positive and gram-negative bacteria and add only 5.2 wt% to the fabric. This nanocoating provides a unique opportunity to impart antibacterial behavior to textiles without harming the environment.

中文翻译：

---

新型植酸基多层纳米涂层抗菌棉

为了使用可再生和对环境无害的成分赋予棉花抗菌性能，使用逐层 (LbL) 组装处理织物。植酸 (PA) 和壳聚糖 (CH) 的交替层从水中沉积。根据美国纺织化学家和染色师协会 (AATCC ) 对涂有四个和八个阴离子 PA 和阳离子 CH 双层 (BL) 的棉布进行了评估，在 CH 溶液中添加或不添加硫酸铜 (II) (CuSO ₄ ) ) TM 100-2019 测试方法。革兰氏阳性金黄色葡萄球菌和革兰氏阴性肺炎克雷伯菌用于确定将铜盐添加到 CH 网络中是否会提高抗菌功效。铜离子成功地结合到使用 LbL 组装到棉纤维上形成的 CH 网络中。用电子显微镜确定沉积铜离子的位置。PA/CH-硫酸铜 (II) 仅 4 BLs 即可杀死 100% 的革兰氏阳性和革兰氏阴性细菌，并且仅在织物中添加 5.2 wt%。这种纳米涂层提供了一个独特的机会，可以在不损害环境的情况下赋予纺织品抗菌性能。