Rice production is facing serious threats due to frequent outbreak of brown plant hopper, Nilaparvata lugens (Stål.) population. Globally, four biotypes of N. lugens has been recorded, of which biotype-4 is the utmost damaging one prevailing in Asian subcontinents particularly in India. Therefore, effective management of N. lugens requires durable resistant rice variety with multiple resistant traits and genes. Hence, in the present study 268 rice genotypes were screened against N. lugens. From initial screening results, 96 genotypes were selected for further genotyping using 93 molecular markers linked to 26 different N. lugens resistant (R) genes. Genetic analysis revealed the mean genetic diversity of 0.275 and polymorphic information content of 0.240 for all 93 markers. Genetic association through cluster and population structure analysis clearly differentiated the 96 genotypes into three major genetic groups with resistant genotypes grouped in a separate cluster. Likewise, Principal co-ordinate analysis supported the above claim with susceptible, moderately resistant and resistant genotypes accommodated in separate components. Further, genetic variation among population showed a 92% variation among individuals of the population and only 8% between populations. Marker trait association via generalized linear model (GLM) and multi linear model (MLM) picked out five markers for their significant association with different phenotypic parameters such as percent damage, nymphal survival, feeding mark and honeydew excretion. Three markers viz. RM19291 (Bph30), RM28472 (Bph18) and RM28449 (Bph17) were common for both GLM and MLM analysis. These identified resistant genes in rice landrace will serves as the source for development of durable resistant variety against N. lugens.

由于褐飞虱 Nilaparvata lugens (Stål.) 种群的频繁爆发，水稻生产面临严重威胁。在全球范围内，已记录了四种褐飞虱生物型，其中 4 型生物型是亚洲次大陆特别是印度流行的最具破坏性的一种。因此，有效管理褐飞虱需要具有多种抗性性状和基因的持久抗性水稻品种。因此，在本研究中，针对褐飞虱筛选了 268 种水稻基因型。从初步筛选结果中，使用与 26 个不同褐飞虱相关的 93 个分子标记选择了 96 个基因型进行进一步基因分型抗性（R）基因。遗传分析显示所有93个标记的平均遗传多样性为0.275，多态信息含量为0.240。通过聚类和群体结构分析的遗传关联清楚地将 96 种基因型区分为三个主要遗传组，其中抗性基因型分组在一个单独的聚类中。同样，主坐标分析支持上述主张，其中易感、中等抗性和抗性基因型包含在单独的组件中。此外，种群间的遗传变异显示种群个体间的变异率为 92%，而种群间的变异仅为 8%。标记特征关联通过广义线性模型 (GLM) 和多线性模型 (MLM) 挑选出五个标志物，因为它们与不同的表型参数（例如损伤百分比、若虫存活率、摄食标记和蜜露排泄量）显着相关。三个标记，即。RM19291 ( Bph30 )、RM28472 ( Bph18 ) 和 RM28449 ( Bph17 ) 在 GLM 和 MLM 分析中都很常见。这些在水稻地方品种中鉴定的抗性基因将作为开发针对褐飞虱的持久抗性品种的来源。