Previous studies conducted in our laboratory, which resorted to 40-day oral exposures to BDE-47 in specific developmental windows of medaka (Oryzias latipes) did not evidence effects on growing or breeding periods. In this new study, full life cycle (i.e. 140-day) dietary exposure to 1000 ng of BDE-47/g was performed with medaka to evaluate effects on growth and reproduction (i.e. fecundity, fertility, hatchability), and to analyze the bioacumulated BDE-47 in and transferred to offspring. No significant effects were observed for the biometric analyses during the growth and maturation periods and no biased sex ratios were found. Reproductive capacity was not affected by the presence of BDE-47 in diet. There was no evidence for apparent effects from parental exposure during embryo and eleutheroembryo development. The analytical results revealed steady BDE-47 bioaccumulation during the growing period, which remained in the reproductive phase in males, and a decreasing tendency was noted in females. These lowering BDE-47 levels in females coincided with the detected BDE-47 levels offloaded in embryos. In the 10-day-old post-hatch larvae, the BDE-47 concentrations dropped to comparatively lower values than the concentrations detected in parents. This finding suggests an efficient metabolic process in the eleutheroembryonic and post-eleutheroembryonic phases. Our 140-day dietary approach found no BDE-47 effects on medaka growth and reproduction, or in early progeny stages despite effective bioaccumulation and maternal transfer.

以前在我们实验室进行的研究是在青鳉 ( Oryzias latipes ) 的特定发育窗口中对 BDE-47 进行 40 天的口服暴露) 没有证据表明对生长或繁殖期有影响。在这项新研究中，对青鳉进行了 1000 纳克 BDE-47/g 的全生命周期（即 140 天）膳食暴露，以评估对生长和繁殖的影响（即繁殖力、生育力、孵化率），并分析生物累积BDE-47 进入并转移给后代。在生长和成熟期间没有观察到生物特征分析的显着影响，也没有发现有偏见的性别比例。饮食中 BDE-47 的存在不会影响生殖能力。没有证据表明在胚胎和刺五加胚胎发育过程中父母接触有明显影响。分析结果表明，BDE-47 在生长期保持稳定，雄性仍处于生殖阶段，雌性呈下降趋势。雌性 BDE-47 水平的降低与胚胎中检测到的 BDE-47 水平相吻合。在孵化后 10 日龄的幼虫中，BDE-47 浓度下降到比在亲本中检测到的浓度更低的值。这一发现表明在eleutheroembryonic 和post-eleutheroembryonic 阶段的有效代谢过程。我们的 140 天饮食方法发现 BDE-47 对青鳉的生长和繁殖没有影响，或者在后代早期阶段没有发现，尽管有效的生物积累和母体转移。这一发现表明在eleutheroembryonic 和post-eleutheroembryonic 阶段的有效代谢过程。我们的 140 天饮食方法发现 BDE-47 对青鳉的生长和繁殖没有影响，或者在后代早期阶段没有发现，尽管有效的生物积累和母体转移。这一发现表明在eleutheroembryonic 和post-eleutheroembryonic 阶段的有效代谢过程。我们的 140 天饮食方法发现 BDE-47 对青鳉的生长和繁殖没有影响，或者在后代早期阶段没有发现，尽管有效的生物积累和母体转移。