The gasotransmitter hydrogen sulfide (H₂S) is an important biological mediator, playing an essential role in many physiological and pathological processes. It is produced by transsulfuration – an evolutionarily highly conserved pathway for the metabolism of sulfur-containing amino acids methionine and cysteine. Cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE) enzymes play a central role in cysteine metabolism and H₂S production. Here we investigated the fitness components (longevity, stress resistance, viability of preimaginal stages, and reproductive function parameters) in D. melanogaster lines containing deletions of the CBS and CSE genes. Surprisingly, in most tests, CSE deletion improved, and CBS worsened the fitness. Lines with deletion of both CBS and CSE demonstrated better stress resistance and longevity than lines with single CBS deletion. At the same time, deletion of both CBS and CSE genes causes more serious disturbances of reproductive function parameters than single CBS deletion. Thus, a complex interaction of H₂S-producing pathways and cellular stress response in determining the lifespan and fitness components of the whole organism was revealed.

气体递质硫化氢（H ₂ S）是一种重要的生物介质，在许多生理和病理过程中发挥着重要作用。它是通过转硫反应产生的——一种进化上高度保守的含硫氨基酸蛋氨酸和半胱氨酸代谢途径。胱硫醚-β-合酶 (CBS) 和胱硫醚-γ-裂合酶 (CSE) 酶在半胱氨酸代谢和 H ₂ S 产生中起核心作用。在这里，我们研究了含有CBS和CSE基因缺失的黑腹果蝇品系中的适应性成分（寿命、抗逆性、成虫前阶段的生存能力和生殖功能参数） 。令人惊讶的是，在大多数测试中，CSE删除得到改善，CBS使适应度恶化。CBS和CSE都缺失的品系比单个CBS缺失的品系表现出更好的抗逆性和寿命。同时，CBS和CSE基因的缺失导致生殖功能参数的紊乱比单独的CBS缺失更严重。_{因此，揭示了H 2} S 产生途径和细胞应激反应在确定整个生物体的寿命和适应性成分方面的复杂相互作用。