In this study, quality evaluation (QE) of 40 batches of decoction pieces of Gardeniae Fructus (GF) produced by different manufacturers of herbal pieces was performed by qualitative analysis of the HPLC fingerprint and ultra-fast liquid chromatography (UFLC)-triple-Q-TOF-MS/MS combined with quantitative analysis of multiple components, which we established previously for QE of traditional medicine. First, HPLC fingerprints of 40 samples were determined, and the common peaks in the reference fingerprint were assigned. Second, the components of the common peaks in the HPLC fingerprints were identified by UFLC-triple-Q-TOF-MS/MS. Finally, the contents of the components confirmed by reference substances were measured. The results showed that there were 28 common peaks in the HPLC fingerprints of 40 samples. The components of these 28 common peaks were identified as 13 iridoids, 4 crocins, 7 monocyclic monoterpenoids, 3 organic acids, and 1 flavonoid. Of these, a total of 12 components, including 7 iridoids of geniposide, shanzhiside, geniposidic acid, deacetyl asperulosidic acid methyl ester, gardenoside, scandoside methyl ester, and genipin gentiobioside, 2 crocins such as crocin I and crocin II, 1 monocyclic monoterpenoid of jasminoside B, 1 organic acid of chlorogenic acid, and 1 flavonoid of rutin, were unambiguously identified by comparison with reference substances. There were certain differences in the contents of these 12 components among 40 samples. The geniposide content ranged from 37.917 to 72.216 mg/g, and the total content of the 7 iridoids ranged from 59.931 to 94.314 mg/g.

中文翻译：

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基于HPLC指纹图谱和Triple-Q-TOF-MS/MS定性分析并结合12种代表性成分定量分析的栀子饮片质量评价

本研究通过HPLC指纹图谱和超快速液相色谱（UFLC）-triple-Q定性分析，对不同厂家生产的40批栀子（GF）饮片进行质量评价（QE）。 -TOF-MS/MS 结合多种成分的定量分析，这是我们之前为传统医学的 QE 建立的。首先，测定了 40 个样品的 HPLC 指纹图谱，并确定了参考指纹图谱中的共同峰。其次，通过 UFLC-triple-Q-TOF-MS/MS 对 HPLC 指纹图谱中常见峰的成分进行了鉴定。最后，测定由对照品确认的成分的含量。结果表明，40个样品的HPLC指纹图谱共有28个共同峰。这28个共同峰的成分被鉴定为环烯醚萜类13个、藏红花素4个、单环单萜类7个、有机酸3个、黄酮类1个。其中，共有12个成分，包括栀子苷、山栀苷、栀子苷酸、去乙酰基阿霉素酸甲酯、栀子苷、斯堪多苷甲酯、京尼平龙胆双苷7个环烯醚萜类化合物，藏红花素I、藏红花素II等2个藏红花素，1个单环单萜茉莉苷B、绿原酸有机酸1个、芦丁类黄酮1个，与对照品比对，明确鉴定。40份样品中这12种成分的含量存在一定差异。栀子苷含量为37.917～72.216 mg/g，7种环烯醚萜总含量为59.931～94.314 mg/g。7 种单环单萜、3 种有机酸和 1 种黄酮类化合物。其中，共有12个成分，包括栀子苷、山栀苷、栀子苷酸、去乙酰基阿霉素酸甲酯、栀子苷、斯堪多苷甲酯、京尼平龙胆双苷7个环烯醚萜类化合物，藏红花素I、藏红花素II等2个藏红花素，1个单环单萜茉莉苷B、绿原酸有机酸1个、芦丁类黄酮1个，与对照品比对，明确鉴定。40份样品中这12种成分的含量存在一定差异。栀子苷含量为37.917～72.216 mg/g，7种环烯醚萜总含量为59.931～94.314 mg/g。7 种单环单萜、3 种有机酸和 1 种黄酮类化合物。其中，共有12个成分，包括栀子苷、山栀苷、栀子苷酸、去乙酰基阿霉素酸甲酯、栀子苷、斯堪多苷甲酯、京尼平龙胆双苷7个环烯醚萜类化合物，藏红花素I、藏红花素II等2个藏红花素，1个单环单萜茉莉苷B、绿原酸有机酸1个、芦丁类黄酮1个，与对照品比对，明确鉴定。40份样品中这12种成分的含量存在一定差异。栀子苷含量为37.917～72.216 mg/g，7种环烯醚萜总含量为59.931～94.314 mg/g。脱乙酰基阿司匹林酸甲酯、栀子苷、斯堪多苷甲酯和京尼平龙胆二苷、藏红花素 I 和藏红花素 II 等 2 种藏红花素、茉莉苷 B 的 1 种单环单萜、绿原酸的有机酸 1 和芦丁的 1 种黄酮类化合物，通过以下方法明确鉴定与参考物质的比较。40份样品中这12种成分的含量存在一定差异。栀子苷含量为37.917～72.216 mg/g，7种环烯醚萜总含量为59.931～94.314 mg/g。脱乙酰基阿司匹林酸甲酯、栀子苷、斯堪多苷甲酯和京尼平龙胆二苷、藏红花素 I 和藏红花素 II 等 2 种藏红花素、茉莉苷 B 的 1 种单环单萜、绿原酸的有机酸 1 和芦丁的 1 种黄酮类化合物，通过以下方法明确鉴定与参考物质的比较。40份样品中这12种成分的含量存在一定差异。栀子苷含量为37.917～72.216 mg/g，7种环烯醚萜总含量为59.931～94.314 mg/g。通过与参考物质的比较明确地确定。40份样品中这12种成分的含量存在一定差异。栀子苷含量为37.917～72.216 mg/g，7种环烯醚萜总含量为59.931～94.314 mg/g。通过与参考物质的比较明确地确定。40份样品中这12种成分的含量存在一定差异。栀子苷含量为37.917～72.216 mg/g，7种环烯醚萜总含量为59.931～94.314 mg/g。