Cultivation in agroforestry system represents an alternative for agroecological production for plants with economic potential, such as medicinal plants. The productive efficiency of this system requires adequate and efficient management strategies for the different intercropped species. The present study aimed to evaluate shoot biomass and production of secondary metabolites (essential oil and flavonoids) in different harvest periods (6, 12 and 18 months) from three medicinal species of economic interest, Mikania laevigata, Varronia curassavica, and Fridericia chica, organically fertilized (organic compost and vermicompost) and grown in agroforestry system with different plant density (spacing). The association of medicinal species with arbuscular mycorrhizal fungi was also evaluated and the chemical soil properties were analyzed before and after fertilization. The experiment was conducted according to the randomized block design, with four blocks and plots subdivided over time, considering the three harvest periods (6, 12 and 18 months). The cultivation of M. laevigata, V. curassavica and F. chica intercropped with Hevea brasiliensis and Theobroma cacao produced enough leaf biomass for the productive viability of medicinal plants in agroforestry systems. Fertilization with vermicompost is the most indicated for dry shoot biomass production, while for higher content of essential oil and flavonoids in the leaves of medicinal plants, the use of organic compost is more indicated. Regarding the harvest period, Varronia curassavica showed the highest dry shoot biomass and essential oil yield at 18 months, while for Mikania laevigata and Fridericia chica, highest production occurred at 12 months. The agroforestry system with lower density of cocoa trees enabled greater dry biomass production from the three medicinal plants and higher yield of essential oil from M. laevigata and V. curassavica. In order to obtain higher contents of flavonoids in F. chica, cultivation in agroforestry system with higher density of cocoa trees is recommended. The cultivation of these three medicinal plants in AFS is a viable alternative for the production of secondary metabolites of pharmaceutical interest.

农林业系统中的栽培代表了具有经济潜力的植物（例如药用植物）的农业生态生产的替代方案。该系统的生产效率需要对不同的间作物种采取适当和有效的管理策略。本研究旨在评估三种具有经济价值的药用物种（ Mikania laevigata、Varronia curassavica和Fridericia chica）在不同收获期（6、12 和 18 个月）的枝条生物量和次生代谢物（精油和类黄酮）的产量，有机施肥（有机堆肥和蚯蚓堆肥），并在不同植物密度（间距）的农林业系统中生长。还评估了药用物种与丛枝菌根真菌的关联，并分析了施肥前后的土壤化学性质。该实验是根据随机区组设计进行的，考虑到三个收获期（6、12 和 18 个月），随着时间的推移细分了四个区组和地块。M. laevigata、V. curassavica和F. chica与巴西橡胶树和可可树间作栽培为农林业系统中药用植物的生产活力产生了足够的叶生物量。用蚯蚓堆肥施肥最适合生产干枝生物质，而对于药用植物叶子中较高含量的精油和类黄酮，更适合使用有机堆肥。在收获期方面，花楸树在 18 个月时表现出最高的干枝生物量和精油产量，而对紫薇和菊花在 12 个月时产量最高。可可树密度较低的农林业系统使三种药用植物的干生物量产量更高，M. laevigata和M. laevigata的精油产量更高五. curassavica。为了获得更高的黄酮类化合物含量，建议在可可树密度较高的农林复合系统中种植。在 AFS 中种植这三种药用植物是生产具有药用价值的次级代谢物的可行替代方案。