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Effect of paeonol against bacterial growth, biofilm formation and dispersal of Staphylococcus aureus and Listeria monocytogenes in vitro
Biofouling ( IF 2.7 ) Pub Date : 2022-02-23 , DOI: 10.1080/08927014.2022.2045014
Qiao Zeng 1, 2 , Yuting Fu 1 , Min Yang 1 , Ting Wang 1 , Ying Wang 3 , Shenghua Lv 2 , Weidong Qian 1
Affiliation  

Abstract

Previous studies have demonstrated the antibacterial activity of paeonol against bacterial pathogens, but its anti-biofilm activities against Staphylococcus aureus and Listeria monocytogenes remain largely unexplored. Here, the antibacterial and anti-biofilm activities of paeonol against S. aureus and L. monocytogenes were examined using the crystal violet staining assay (CVSA), field emission scanning electron microscopy (FESEM), and confocal laser scanning microscopy (CLSM) analysis. Paeonol effectively inhibited the growth of S. aureus and L. monocytogenes with a minimum inhibitory concentration (MIC) of 500 and 125 μg ml−1, respectively, and disrupted the integrity of cell membranes. Moreover, sub-MIC paeonol exhibited an inhibitory effect on the attachment of S. aureus and L. monocytogenes to the abiotic surface and biofilm formation. Further, paeonol effectively destroyed cell membranes within biofilms, and dispersed mature biofilms of both strains. The results indicate that paeonol might be a promising antibacterial and anti-biofilm agent for combating infections caused by S. aureus and L. monocytogenes.



中文翻译:

丹皮酚对金黄色葡萄球菌和单核细胞增生李斯特菌的体外生长、生物膜形成和扩散的影响

摘要

以前的研究已经证明了丹皮酚对细菌病原体的抗菌活性,但其对金黄色葡萄球菌单核细胞增生李斯特菌的抗生物膜活性在很大程度上仍未得到探索。在这里,使用结晶紫染色法 (CVSA)、场发射扫描电子显微镜 (FESEM) 和共聚焦激光扫描显微镜 (CLSM) 分析检查了丹皮酚对金黄色葡萄球菌单核细胞增生李斯特菌的抗菌和抗生物膜活性。丹皮酚有效抑制金黄色葡萄球菌单核细胞增生李斯特菌的生长,最低抑菌浓度 (MIC) 分别为 500 和 125 μg ml -1,分别破坏细胞膜的完整性。此外,亚MIC 丹皮酚对金黄色葡萄球菌单核细胞增生李斯特菌在非生物表面的附着和生物膜形成具有抑制作用。此外,丹皮酚有效地破坏了生物膜内的细胞膜,并分散了两种菌株的成熟生物膜。结果表明,丹皮酚可能是一种很有前途的抗菌剂和抗生物膜剂,可用于对抗由金黄色葡萄球菌单核细胞增生李斯特菌引起的感染。

更新日期:2022-02-23
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