Animal Nutrition ( IF 6.3 ) Pub Date : 2022-02-17 , DOI: 10.1016/j.aninu.2022.02.001 Shumin Zhang 1 , Jinbiao Zhao 1 , Jinhua Hu 1 , Hengxun He 1 , Yihan Wei 1 , Linbao Ji 1 , Xi Ma 1
The offspring of super-multiparous sows face problems such as decreased growth performance, poor meat quality and even diseases in animal husbandry. Gama-aminobutyric acid (GABA) has long been known to promote growth and suppress inflammation, but little is known about the mechanisms. A total of 72 growing-finishing pigs from the 8th generation were randomly allotted to 2 groups with 6 replicates per treatment to receive a corn–soybean basal diet or the basal diet supplemented 20 mg/kg GABA for 60 d. After the animal-trial period, samples of serum and liver were collected for further analysis. Additionally, a lipopolysaccharide (LPS)-induced inflammatory model using HepG2 cells was established to explore the role of GABA on regulating hepatic inflammation. The results indicated that inflammatory cell infiltration occurs in the liver of progeny of super-multiparous sows, and dietary supplementation with GABA influenced liver morphology, increased activities of antioxidant enzymes and decreased the expression abundance of pro-inflammatory cytokines, including tumor necrosis factor-α (TNFα) and interleukin (IL)-1β, in the liver of growing-finishing pigs (P < 0.05). In addition, GABA supplementation increased mRNA expressions of peroxisome proliferator-activated receptor γ (PPARγ) and GABA receptors (GABARs), and reduced the expression of toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling (P < 0.05). Additionally, an in vitro experiment demonstrated that GABA decreased the expressions of hepatic TLR4/NF-κB signaling via activating GABARs under LPS-stress (P < 0.05). In summary, liver injury may affect the growth performance of growing-finishing pigs by changing hepatic mitochondrial metabolism, the expression of pro-inflammatory cytokines and TLR4/NF-κB pathway and that GABA supplementation has a restorative effect by acting on GABARs.
中文翻译:
Gama-氨基丁酸 (GABA) 通过 GABA 受体/TLR4/NF-κB 通路减轻超多产母猪产生的生长肥育猪的肝脏炎症
超多产母猪的后代面临着生长性能下降、肉质差甚至畜牧业疾病等问题。众所周知,伽马氨基丁酸 (GABA) 可促进生长和抑制炎症,但对其机制知之甚少。将第 8 代共 72 头生长肥育猪随机分为 2 组,每个处理 6 个重复,接受玉米-大豆基础日粮或添加 20 mg/kg GABA 的基础日粮 60 d。动物试验期结束后,收集血清和肝脏样本进行进一步分析。此外,利用HepG2细胞建立了脂多糖(LPS)诱导的炎症模型,以探索GABA在调节肝脏炎症中的作用。生长肥育猪肝脏中的TNFα ) 和白细胞介素 ( IL ) -1β ( P < 0.05)。此外,补充 GABA 可增加过氧化物酶体增殖物激活受体 γ ( PPARγ ) 和 GABA 受体 ( GABARs ) 的 mRNA 表达,并降低 toll 样受体 4 (TLR4)/核因子-κB (NF-κB) 信号的表达。P < 0.05)。此外,一项体外实验表明,GABA 在 LPS 应激下通过激活 GABARs 降低肝脏 TLR4/NF-κB 信号通路的表达 ( P < 0.05)。综上所述,肝损伤可能通过改变肝脏线粒体代谢、促炎细胞因子的表达和TLR4/NF-κB通路影响生长肥育猪的生长性能,而补充GABA通过作用于GABARs具有恢复作用。