Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system. However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1. Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive. In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly. Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively. It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression. Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1. Significantly, Ginsenoside Rb1 loaded on micro-nano HAp/silk could facilitate osteogenesis and angiogenesis. All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor’s expression of BMSCs. Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.

人参的有效成分人参皂甙 Rb1 已被证明在改善免疫系统方面发挥着有利的作用。然而，关于人参皂甙Rb1的成骨和血管生成作用的研究很少。此外，如何建立人参皂甙Rb1的递送系统及其在骨缺损中的修复能力仍然是个未知数。本研究首先评价了人参皂甙Rb1在大鼠BMSCs细胞活力、增殖、凋亡、成骨基因表达、ALP活性等方面的作用。然后，制备微纳米HAp颗粒与蚕丝结合，建立人参皂甙Rb1的递送系统，并通过连续荧光标记评估载于微纳HAp/蚕丝上的人参皂甙Rb1对大鼠颅骨缺损模型的成骨和血管生成作用，和组织学分析，分别。结果表明，人参皂甙 Rb1 可以维持细胞活力，显着增加 ALP 活性、成骨和血管生成基因的表达。同时，与蚕丝结合的微纳米HAp颗粒被顺利制备，是人参皂甙Rb1的递送载体。值得注意的是，负载在微纳米 HAp/丝上的人参皂甙 Rb1 可以促进成骨和血管生成。所有结果提示人参皂甙Rb1可以增强骨髓间充质干细胞的成骨分化和血管生成因子的表达。此外，微纳米HAp与蚕丝结合可作为人参皂甙Rb1修复骨缺损的载体。与蚕丝结合的微纳米HAp颗粒制备顺利，是人参皂甙Rb1的递送载体。值得注意的是，负载在微纳米 HAp/丝上的人参皂甙 Rb1 可以促进成骨和血管生成。所有结果提示人参皂甙Rb1可以增强骨髓间充质干细胞的成骨分化和血管生成因子的表达。此外，微纳米HAp与蚕丝结合可作为人参皂甙Rb1修复骨缺损的载体。与蚕丝结合的微纳米HAp颗粒制备顺利，是人参皂甙Rb1的递送载体。值得注意的是，负载在微纳米 HAp/丝上的人参皂甙 Rb1 可以促进成骨和血管生成。所有结果提示人参皂甙Rb1可以增强骨髓间充质干细胞的成骨分化和血管生成因子的表达。此外，微纳米HAp与蚕丝结合可作为人参皂甙Rb1修复骨缺损的载体。