Simple and rapid imaging and analysis of 2D and 3D cell culture compatible with miniaturized arrays of nanoliter droplets are essential for high-throughput screening and personalized medicine applications. In this study, we have developed a simple one-step, cost-effective and sensitive colorimetric method for the analysis of cell viability in 2D and 3D cell cultures on a nanoliter droplet microarray. The method utilizes a flatbed document scanner that detects a color change in response to cell metabolism in nanoliter droplets with high sensitivity in a single step without the need for expensive specialized equipment. This new nanoliter-based method is faster and more sensitive than equivalent methods using multi-well plate assays. The method detects quantifiable signal from as few as 10 cells and requires only 5 min. This is 2.5 to 10-fold more sensitive and 12 times faster than the same assay in multi-well plates. The method is simple, affordable, fast and sensitive. It can be used for various applications including high-throughput cell-based and biochemical screenings.

与纳升液滴微型阵列兼容的 2D 和 3D 细胞培养物的简单快速成像和分析对于高通量筛选和个性化医疗应用至关重要。在这项研究中，我们开发了一种简单的一步法、经济高效且灵敏的比色法，用于在纳升液滴微阵列上分析 2D 和 3D 细胞培养物中的细胞活力。该方法使用平板文档扫描仪，该扫描仪在一个步骤中以高灵敏度检测响应于纳升液滴中细胞代谢的颜色变化，而无需昂贵的专用设备。这种新的基于纳升的方法比使用多孔板测定的等效方法更快、更灵敏。该方法检测来自少至 10 个细胞的可量化信号，并且只需要 5 分钟。这是2。与多孔板中的相同检测相比，灵敏度提高 5 到 10 倍，速度提高 12 倍。该方法简单、经济、快速、灵敏。它可用于各种应用，包括基于细胞的高通量和生化筛选。