When pesticides reach the aquatic environment, they can distribute in water and sediment, increasing the risks to benthic organisms, such as amphipods that play a key role in the aquatic food webs. Thus, the present study assessed the consequences of exposure to the insecticide fipronil and herbicide 2,4-D (alone and in mixture) on biochemical markers, feeding rates and the partial life-cycle of Hyalella meinerti. Three concentrations of fipronil (0.1, 0.3, and 0.7 µg L⁻¹) and 2,4-D (19, 124, and 654 µg L⁻¹), and six mixture combinations were assessed. The first experiment was carried out with males and females separately assessing the feeding rates, total carbohydrate content, and lipid profile. The second (partial life-cycle) lasted 49 days, and the survival, growth, and reproductive endpoints were assessed. Both pesticides and their mixture caused decreases in feeding rates, mainly in females. Females also suffered a change in the total carbohydrate content. In addition, there were changes in the percentage of triacylglycerol and phospholipids in males and females. Furthermore, alterations occurred in the percentual of triacylglycerol and phospholipids to both sexes. In the second experiment, fipronil and the mixtures caused decreases in the survival of H. meinerti over time. Exposure to 2,4-D, fipronil, and their mixture impaired the 28-day growth leading to biomass loss ranging from 17–23%, 54–60%, and 22–49%, respectively. The insecticide and mixture caused increases in time to sexual maturation of up to 10 and 6 days, respectively, and reduced the number of formed couples. Furthermore, fipronil decreased reproduction up to 36 times and no juveniles were produced in some mixture combinations. In addition, the pesticides on isolation decreased the juvenile size. Finally, exposure to both pesticides, alone or in a mixture, decreased the intrinsic rate of population growth. The results were observed in concentrations already quantified in water bodies, with risks for ecosystems functioning due to the importance of amphipods in aquatic ecosystems.

当杀虫剂到达水生环境时，它们会分布在水和沉积物中，从而增加了底栖生物的风险，例如在水生食物网中发挥关键作用的片脚类动物。因此，本研究评估了暴露于杀虫剂氟虫腈和除草剂 2,4-D（单独和混合）对生化标志物、摄食率和Hyalella meinerti部分生命周期的影响。三种浓度的氟虫腈（0.1、0.3 和 0.7 µg L ^-1）和 2,4-D（19、124 和 654 µg L ^-1)，并评估了六种混合物组合。第一个实验是用雄性和雌性分别评估摄食率、总碳水化合物含量和脂质分布进行的。第二个（部分生命周期）持续 49 天，评估生存、生长和生殖终点。杀虫剂及其混合物均导致采食率下降，主要是雌性。女性的总碳水化合物含量也发生了变化。此外，男性和女性中甘油三酯和磷脂的百分比也发生了变化。此外，两性中甘油三酯和磷脂的百分比发生了变化。在第二个实验中，氟虫腈及其混合物导致H. meinerti存活率下降随着时间的推移。暴露于 2,4-D、氟虫腈及其混合物会损害 28 天的生长，导致生物量损失分别为 17-23%、54-60% 和 22-49%。杀虫剂和混合物导致性成熟时间分别增加了 10 天和 6 天，并减少了成对的数量。此外，氟虫腈减少了高达 36 倍的繁殖，并且在某些混合物组合中没有产生幼鱼。此外，隔离时的杀虫剂减小了幼体的大小。最后，暴露于两种农药，单独或混合使用，会降低人口的内在增长率。结果是在水体中已经量化的浓度中观察到的，由于端足类动物在水生生态系统中的重要性，生态系统功能面临风险。