The objective of this experiment was to evaluate the effect of a combination of microencapsulated essential oils and organic acids (MOA) on growth performance, immuno-antioxidant status, intestinal barrier function and microbial structure of the hindgut in piglets. A total of 120 piglets (Duroc × [Landrace × Yorkshire]; weighted 7.66 ± 1.79 kg, weaned at d 28) were randomly selected and allocated to 3 treatments with 4 replicates per group and 10 piglets per replicate according to the initial body weight and gender. The dietary treatments were as follows: 1) basal diet (Ctrl); 2) Ctrl + chlortetracycline (75 mg/kg) (AGP); 3) Ctrl+ MOA (1500 mg/kg). The experiment period was lasted for 21 d. Compared to the Ctrl group, dietary supplemented MOA alleviated (P < 0.05) the diarrhea rate from d 12 to 21, enhanced (P < 0.05) the concentration of serum interlukin-10 and glutathione peroxidase in piglets on d 11 after weaning and serum superoxide dismutase in 21-day piglets. The MOA group also improved (P < 0.05) the apparent digestibility of dry matter (DM), organic matter (OM) and gross energy (GE), up-regulated (P < 0.05) the mRNA expression level of occludin, claudin-1 and mucin-2 in ileum and increased (P < 0.05) the contents of propionic and butyric acids in the cecum of piglets. The MOA group modulated the cecal and colonic microbial community structure and increased (P < 0.05) the abundance of Faecalibacterium and Muribaculaceae in cecum and Streptococcus and Weissella in colon. Additionally, AGP group decreased (P < 0.05) apparent digestibility of DM, OM and GE as well as down-regulated (P < 0.05) relative gene expression level of claudin-1 in duodenum and jejunum, ZO-1 and mucin-1 in jejunum of piglets. In summary, dietary supplemented MOA alleviated diarrhea and improved nutrient apparent digestibility in piglets via enhancing immuno-antioxidant properties, increasing digestive enzyme activity, up-regulating the expression of intestinal barrier-related genes, and modifying the microbial community structure of the cecum and colon. Therefore, dietary supplementation with MOA as an alternative to antibiotics was feasible to improve intestinal health of piglets in practical production.

中文翻译：

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微胶囊精油与有机酸结合可提高免疫抗氧化能力和肠道屏障功能，并调节仔猪后肠微生物群落

本实验的目的是评估微胶囊精油和有机酸 (MOA) 组合对仔猪后肠的生长性能、免疫抗氧化状态、肠道屏障功能和微生物结构的影响。随机选取120头仔猪（Duroc × [Landrace × Yorkshire]；体重7.66±1.79 kg，在第28天断奶）并根据初始体重和初始体重分配到3个处理，每组4个重复，每个重复10头仔猪。性别。饮食处理如下： 1）基础饮食（Ctrl）；2）Ctrl+金霉素（75mg/kg）（AGP）；3) Ctrl+ MOA (1500 毫克/公斤)。试验期持续21 d。与 Ctrl 组相比，膳食补充 MOA 在第 12 天至第 21 天的腹泻率减轻（P < 0.05），增强（P < 0.05）。05)断奶后第11天仔猪血清interlukin-10和谷胱甘肽过氧化物酶浓度和21天仔猪血清超氧化物歧化酶浓度。MOA组还提高（P < 0.05）干物质（DM）、有机物（OM）和总能量（GE）的表观消化率，上调（P < 0.05）occludin、claudin-1的mRNA表达水平回肠黏蛋白2和回肠黏蛋白2增加（P < 0.05）仔猪盲肠中丙酸和丁酸的含量。MOA组调节盲肠和结肠微生物群落结构并增加（P <0.05）盲肠中粪杆菌和Muribaculaceae以及结肠中链球菌和Weissella的丰度。此外，AGP 组 DM、OM 和 GE 的表观消化率降低（P < 0.05）并下调（P < 0.05）。05)仔猪十二指肠和空肠中claudin-1、ZO-1和mucin-1的相对基因表达水平。总之，日粮中添加 MOA 通过增强免疫抗氧化特性、增加消化酶活性、上调肠道屏障相关基因的表达以及改变盲肠和结肠的微生物群落结构来缓解仔猪腹泻和改善养分表观消化率。 . 因此，在实际生产过程中，在日粮中添加 MOA 替代抗生素对改善仔猪肠道健康是可行的。增加消化酶活性，上调肠道屏障相关基因的表达，改变盲肠和结肠的微生物群落结构。因此，在实际生产过程中，在日粮中添加 MOA 替代抗生素对改善仔猪肠道健康是可行的。增加消化酶活性，上调肠屏障相关基因的表达，改变盲肠和结肠的微生物群落结构。因此，在实际生产过程中，在日粮中添加 MOA 替代抗生素对改善仔猪肠道健康是可行的。