High salt concentrations can restrict or inhibit microorganism activity, which can detrimentally influence pollutant degradation. In this study, intertidal wetland sediments (IWS) are introduced into constructed wetlands (CWs) as sources of microorganisms. The richness and evenness of bacterial and archaeal microbial communities in the presence of 150 × 10⁻³ m NaCl are evaluated by high-throughput sequencing. The distinction of dominant functional microorganisms in CWs with and without IWS is also analyzed. Anaerolineales and thermomicrobia are the dominant microorganisms in IWS–CWs in the saline condition and are related to methane production. The abundance of Alphaproteobacteria, Myxococcales, and Xanthomonadales, related to dehydrogenation, is relatively high in IWS–CWs. The addition of IWS induces further production of Anaerolineaceae and Geobacteraceae, which is positively correlated with the phosphorus-accumulating and sulfate-reducing stages. The dominant archaea phyla in IWS–CWs in the saline condition are Thaumarchaeota (87.5%) and Euryarchaeota (12.2%), which are involved in ammonia-oxidizing and methane-producing processes, respectively. These functional microbial communities are beneficial to the degradation and transformation of pollutants in the CWs. Thus, IWS with various halophilic and pollutant-degrading microorganisms can be considered effective microbial sources to enhance the pollutant removal abilities of CWs.

中文翻译：

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NaCl胁迫下微生物强化人工湿地细菌和古菌群落特征

高盐浓度会限制或抑制微生物活动，从而对污染物降解产生不利影响。在这项研究中，潮间带湿地沉积物（IWS）被引入人工湿地（CWs）作为微生物来源。^{150 × 10 -3} m存在下细菌和古菌微生物群落的丰富度和均匀度通过高通量测序评估 NaCl。还分析了具有和不具有 IWS 的 CW 中优势功能微生物的区别。Anaerolineales 和 thermomicrobia 是盐水条件下 IWS-CWs 中的主要微生物，并且与甲烷的产生有关。在 IWS-CW 中，与脱氢有关的 Alphaproteobacteria、Myxococcales 和 Xanthomonadales 的丰度相对较高。IWS的加入诱导了厌氧菌科和地杆菌科的进一步产生，这与磷积累和硫酸盐还原阶段呈正相关。盐水条件下 IWS-CWs 中的主要古菌门是 Thaumarchaeota (87.5%) 和 Euryarchaeota (12.2%)，它们分别参与氨氧化和甲烷产生过程。这些功能性微生物群落有利于CWs中污染物的降解和转化。因此，具有各种嗜盐和污染物降解微生物的 IWS 可以被认为是有效的微生物来源，以提高 CWs 的污染物去除能力。