The aim of our study was to illustrate the role of circular RNA 0120175 (circ_0120175) and its associated mechanism in laryngeal squamous cell carcinoma (LSCC) development. The abundance of circ_0120175, microRNA-330-3p (miR-330-3p) and solute carrier family 7, membrane 11 (SLC7A11) messenger RNA and protein was measured by quantitative real time polymerase chain reaction and Western blot assay. Cell proliferation, apoptosis, migration and invasion were assessed by cell counting kit-8 assay, flow cytometry and transwell migration and invasion assays, respectively. The interaction between miR-330-3p and circ_0120175 or SLC7A11 was confirmed by dual-luciferase reporter assay. Murine xenograft model was established to test the function of circ_0120175 in tumor growth in vivo. Circ_0120175 abundance was aberrantly increased in LSCC tissues and cell lines, and LSCC patients with high level of circ_0120175 were associated with advanced tumor staging, lymph node metastasis and short survival time. Circ_0120175 interference suppressed cell proliferation, migration and invasion and induced cell apoptosis of LSCC cells. Circ_0120175 could sponge and negatively regulate miR-330-3p expression in LSCC cells. The addition of anti-miR-330-3p partly reversed circ_0120175 knockdown-induced effects in LSCC cells. SLC7A11 bound to miR-330-3p. Circ_0120175 enhanced the abundance of SLC7A11 through sponging miR-330-3p in LSCC cells. Circ_0120175 silencing-mediated influences in LSCC cells were partly counteracted by the overexpression of SLC7A11. Circ_0120175 interference notably suppressed xenograft tumor growth in vivo. Circ_0120175 promoted proliferation, migration and invasion while impeded cell apoptosis of LSCC cells through miR-330-3p/SLC7A11 axis, which provided novel therapeutic targets for LSCC.

我们研究的目的是阐明环状 RNA 0120175 (circ_0120175) 的作用及其在喉鳞状细胞癌 (LSCC) 发展中的相关机制。circ_0120175、microRNA-330-3p (miR-330-3p) 和溶质载体家族 7、膜 11 (SLC7A11) 信使 RNA 和蛋白质的丰度通过定量实时聚合酶链反应和蛋白质印迹法测定。细胞增殖、凋亡、迁移和侵袭分别通过细胞计数 kit-8 测定、流式细胞术和 transwell 迁移和侵袭测定来评估。miR-330-3p 与 circ_0120175 或 SLC7A11 之间的相互作用通过双荧光素酶报告基因分析得到证实。建立小鼠异种移植模型，检测circ_0120175在体内肿瘤生长中的作用。Circ_0120175 丰度在 LSCC 组织和细胞系中异常升高，且 circ_0120175 水平高的 LSCC 患者与肿瘤分期晚期、淋巴结转移和生存时间短有关。Circ_0120175 干扰抑制了 LSCC 细胞的增殖、迁移和侵袭，并诱导细胞凋亡。Circ_0120175 可以海绵化并负调节 LSCC 细胞中 miR-330-3p 的表达。添加抗 miR-330-3p 部分逆转了 circ_0120175 敲低诱导的 LSCC 细胞的作用。SLC7A11 与 miR-330-3p 结合。Circ_0120175 通过海绵化 miR-330-3p 在 LSCC 细胞中增强 SLC7A11 的丰度。SLC7A11 的过表达部分抵消了 Circ_0120175 沉默介导的 LSCC 细胞影响。Circ_0120175 干扰显着抑制了体内异种移植肿瘤的生长。