Palm oil mill effluent (POME) is a by-product of palm oil production. POME can be considered as harmful waste without any treatment. POME, however, has the potential to be a source of low-cost carbon for lipid production. This study aims to isolate potential oleaginous fungi from POME and to evaluate the ability of lipid production capacity of the purified isolates and commercial oleaginous fungi strains (Aspergillus niger, Aspergillus oryzae and Rhizopus spp.) from POME. POME is converted into lipid via the metabolic pathway of oleaginous fungi, where the lipids can be further trans-esterified into microbial biodiesel. In this study, two fungal strains are successfully isolated from POME. The combination of Bligh and Dyer with Soxhlet extraction results in better lipid extraction efficiency. A. niger has the highest lipid yield from the cultivation on POME. The projected fuel properties of microbial biodiesel from A. niger are within the limits of Biodiesel Standard. Chemical oxygen demand (COD) removal from POME using A. niger is proven to be effective with 70% COD removal. The production of biodiesel from wastes of the palm oil industry may improve the industry's sustainability through the use of nonfood feedstock coupled with simultaneous bioremediation of POME.

中文翻译：

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通过含油真菌生物修复棕榈油厂废水生产生物柴油

棕榈油厂废水 (POME) 是棕榈油生产的副产品。POME 可被视为有害废物，未经任何处理。然而，POME 有可能成为用于脂质生产的低成本碳源。本研究旨在从 POME 中分离出潜在的产油真菌，并评估纯化分离物和商业产油真菌菌株（黑曲霉、米曲霉和根霉）的脂质生产能力。spp.) 来自 POME。POME通过产油真菌的代谢途径转化为脂质，其中脂质可以进一步酯交换成微生物生物柴油。在这项研究中，从 POME 中成功分离出两种真菌菌株。Bligh 和 Dyer 与 Soxhlet 提取的结合可提高脂质提取效率。A. niger在 POME 上培养的脂质产量最高。来自A. niger的微生物生物柴油的预计燃料特性在生物柴油标准的限制范围内。使用A. niger从 POME 中去除化学需氧量 (COD)经证明有效去除 70% 的 COD。从棕榈油行业的废料中生产生物柴油可以通过使用非食品原料以及同时对 POME 进行生物修复来提高行业的可持续性。