Sugarcane crops are dependent on chemicals for maintaining plantations. Therefore, environmental consequences concern adjacent areas that can be affected by contaminants in common use, including pesticides and vinasse (i.e., a by-product from the ethanol industry). This study aimed to evaluate phytotoxicity through two plant bioassays with water from mesocosms contaminated with the herbicide 2,4-D (447.0 μg L⁻¹), the insecticide fipronil (63.5 μg L⁻¹), and sugarcane vinasse (1.3%). First, the germination test (4 d) with Eruca sativa L. assessed water samples collected three times after the contamination (2 h, 14 d, and 30 d), considering germination, shoot, and root growth. The results from this bioassay indicated higher phytotoxicity for 2,4-D as it fully inhibited the shoot and root growth even in low concentrations (0.2 μg L⁻¹). However, no significant effect was reported for fipronil and vinasse. Also, the 2,4-D effects drastically decreased due to an expressive concentration reduction (99.4% after 30 d in mixture with vinasse). Second, the irrigation test with Phaseolus vulgaris L. and Zea mays L. considered shoot and root growth and biomass under 21 days after plants emergence. The herbicide 2,4-D inhibited the initial growth of tested species, especially the roots (up to 45% inhibition). Furthermore, sugarcane vinasse caused harmful effects on plant growth (up to 31% inhibition). Therefore, our data showed that these contaminants could inhibit plant germination and initial growth under our tested conditions. These evaluations can endorse risk assessments and water management in sugarcane crops surrounding areas.

甘蔗作物依赖化学品来维持种植园。因此，环境后果涉及可能受到常用污染物影响的邻近地区，包括杀虫剂和酒糟（即乙醇工业的副产品）。^{本研究旨在通过对来自被除草剂 2,4-D (447.0 μg L -1} )、杀虫剂氟虫腈 (63.5 μg L ^-1 ) 和甘蔗酒糟 (1.3%)污染的水进行两次植物生物测定来评估植物毒性。一、Eruca sativa发芽试验（4 d）L. 评估污染后 3 次收集的水样（2 小时、14 天和 30 天），考虑发芽、枝条和根系生长。该生物测定的结果表明 2,4-D 具有较高的植物毒性，因为即使在低浓度 (0.2 μg L ^-1 ) 下也能完全抑制枝条和根的生长。然而，没有报道氟虫腈和酒糟的显着效果。此外，由于表达浓度降低（与酒糟混合 30 天后 99.4%），2,4-D 效应急剧下降。二、Phaseolus vulgaris L.和Zea mays的灌水试验L. 考虑植物出苗后 21 天的芽和根生长和生物量。除草剂 2,4-D 抑制受试物种的初始生长，尤其是根部（抑制高达 45%）。此外，甘蔗酒糟对植物生长造成有害影响（抑制高达 31%）。因此，我们的数据表明，在我们测试的条件下，这些污染物可以抑制植物的发芽和初始生长。这些评估可以支持甘蔗作物周边地区的风险评估和水资源管理。