During the inspection of vegetable material for EXPO 2015 in Milan (Italy), samples of bark from an unidentified coniferous tree were analysed and screened for the presence of the pinewood nematode, Bursaphelenchus xylophilus, in accordance with the surveillance of European Union to enable early detection and prevent further spread. In this study, nematodes associated with the bark of unidentified coniferous tree were collected and identified by morphometrics and sequencing of ITS, D2-D3 expansion domains of the 28S rRNA and the partial 18S rRNA genes. The sequences obtained and the phylogenetic analyses, using the ITS, D2-D3 expansion domains of the 28S rRNA and the partial 18S rRNA genes, revealed the presence of four Aphelenchidae species. Two of the species recovered were phylogenetically related to Cryptaphelenchus species. One of the two was identified as C. minutus, as it showed morphology and morphometry largely overlapping with Bursaphelenchus minutus, but phylogenetically closely related to Cryptaphelenchus species. The other three species recovered were only molecularly identified herein, by sequencing the ITS and the D2-D3 expansion domains, as Cryptaphelenchus sp. 1 and two Aphelenchoides species.

在检查 2015 年米兰（意大利）世博会的植物材料期间，根据欧盟的监测，对一棵不明针叶树的树皮样本进行分析和筛选是否存在松木线虫松材线虫（Bursaphelenchus xylophilus ），以便及早发现并防止进一步传播。在这项研究中，通过形态计量学和 ITS、28S rRNA 的 D2-D3 扩展域和部分 18S rRNA 基因的测序，收集并鉴定了与未识别针叶树树皮相关的线虫。获得的序列和系统发育分析，使用 28S rRNA 的 ITS、D2-D3 扩展域和部分 18S rRNA 基因，揭示了四种 Aphelenchidae 物种的存在。发现的两个物种在系统发育上与隐翅目物种。两者之一被确定为C. minutus，因为它的形态和形态学与Bursaphelenchus minutu s 大部分重叠，但在系统发育上与Cryptaphelenchus物种密切相关。通过对 ITS 和 D2-D3 扩展结构域进行测序，回收的其他三种物种仅在本文中被分子鉴定为Cryptaphelenchus sp。1 和两种Aphelenchoides物种。