当前位置: X-MOL 学术Eur. J. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
High concentrations of antagonistic bacterial strains from diseased sanqi ginseng rhizosphere suppressed Fusarium root rot
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2022-02-07 , DOI: 10.1007/s10658-022-02463-4
Yaojun Dong 1 , Binbin Tang 1 , Mimi He 1 , Kai Wu 1 , Sixia Yang 1 , Jianfeng Liu 1 , Hong Yang 1 , Changmei Wang 1 , Fang Yin 1 , Wudi Zhang 1 , Lili Wang 2
Affiliation  

Sanqi ginseng Fusarium root rot caused by Fusarium oxysporum, is a serious soil-borne disease that threatens the sustainable development of the sanqi ginseng industry. This study determined the distinct soil bacterial communities from healthy and diseased plant rhizospheres. The ability of beneficial bacteria isolated from the diseased plant rhizosphere with different inoculation concentrations to control Fusarium root rot was investigated. The evenness and Shannon diversity indexes of bacteria in the diseased plant rhizosphere soil were significantly lower than those in the healthy plant rhizosphere soil because the bacterial communities were enriched in the Bacteroidetes and Proteobacteria phyla. The relative abundances of some genera such as Chryseobacterium, Flavobacterium, Stenotrophomonas, and Lysobacter were significantly larger in the diseased plant rhizosphere than in the healthy plant rhizosphere. Two strains, Bacillus sp. KW6 and Lysobacter sp. KW8, isolated from the diseased plant rhizosphere soil exhibited strong antagonistic activity against F. oxysporum, producing chitinase, cellulase, and β-1,3-glucanase. Bacillus sp. KW6 with an inoculation concentration of 108 colony-forming units/g of soil exhibited the largest suppression in the control of sanqi ginseng root rot. The disease incidence of this treatment was only 38.89%, which was 59% lower than that of the control. Sanqi ginseng fresh weight increased with increasing inoculation concentration in treatments applied with antagonistic strains. The amounts of pathogen in stems and roots from treatments inoculated with antagonistic strains were significantly lower than the control.



中文翻译:

病害三七人参根际高浓度拮抗菌抑制镰刀菌根腐病

由尖孢镰刀菌引起的三七人参镰刀菌根腐病是一种严重的土传病害,威胁着三七人参产业的可持续发展。这项研究确定了健康和患病植物根际不同的土壤细菌群落。研究了不同接种浓度从病株根际分离的有益菌对镰刀菌根腐病的防治能力。病株根际土壤中细菌的均匀度和香农多样性指数显着低于健康植物根际土壤,因为细菌群落富含拟杆菌门和变形杆菌门。门。一些属如金黄杆菌属、黄杆菌属、窄食单胞菌属和溶菌属的相对丰度在患病植物根际明显大于健康植物根际。两种菌株,芽孢杆菌属。KW6 和Lysobacter sp。从病株根际土壤中分离得到的KW8对尖孢镰刀菌具有很强的拮抗活性,可产生几丁质酶、纤维素酶和β-1,3-葡聚糖酶。芽孢杆菌 KW6 接种浓度为 10 8菌落形成单位/g土壤对三七人参根腐病的防治表现出最大的抑制作用。该治疗组发病率仅为38.89%,比对照组降低59%。三七人参鲜重随着拮抗菌株处理的接种浓度的增加而增加。接种拮抗菌株处理的茎和根中病原体的数量显着低于对照。

更新日期:2022-02-09
down
wechat
bug