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Diverse phytoplasmas associated with maize bushy stunt disease in Peru
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2022-02-05 , DOI: 10.1007/s10658-022-02470-5
Delia Gamarra Gamarra 1 , Charo Milagros Villar 1 , Gilberto Torres Suarez 1 , Walter Darlyn Ingaruca Esteban 1 , Emerson Clovis Carrasco Lozano 1 , Nicoletta Contaldo 2 , Assunta Bertaccini 2
Affiliation  

Corn plants showing symptoms of midribs chlorosis, and leaf reddening, short internodes, ear proliferation, and plant growth reduction were collected in Peru from fields in nine localities in the provinces of Huancayo, Chupaca, and Jauja in the Junín region, and tested to verify phytoplasma presence and identity. Primers amplifying the phytoplasma ribosomal 16S and ribosomal protein genes were used. The phytoplasma presence was detected in symptomatic samples from all the surveyed areas. The sequencing of the obtained amplicons indicated the presence of ‘Candidatus Phytoplasma asteris’ and ‘Ca. P. pruni’-related strains. A BLASTn search of sequenced genes showed that the two ‘Candidatus Phytoplasma’ strains identified in corn shares 100% and 99.82% identity with the ‘Ca. P. asteris’ strains from maize and 99.92% and 99.55% with ‘Ca. P. pruni’-related strains, respectively. The RFLP analyses allowed to enclose these phytoplasma strains in the 16SrI-B and 16SrIII-J subgroups; however, the two phytoplasmas were, in some cases, present in mixed infection. The 16SrIII-J phytoplasma is for the first time reported associated with the maize bushy stunt disease and this represent a relevant information for the disease epidemiology towards its appropriate management in the affected area.



中文翻译:

与秘鲁玉米丛生矮化病相关的多种植原体

在秘鲁胡宁地区的万卡约省、丘帕卡省和豪哈省的 9 个地方采集玉米植株出现中脉萎黄、叶片发红、节间短、穗部增殖和植物生长减少等症状,并进行测试以验证植原体的存在和身份。使用了扩增植原体核糖体 16S 和核糖体蛋白基因的引物。在所有调查区域的有症状样本中都检测到了植原体的存在。获得的扩增子的测序表明存在' Candidatus Phytoplasma asteris'和' Ca。P. pruni '相关菌株。对已测序基因的BLASTn搜索表明,这两个在玉米中鉴定的植原体菌株与 ' Ca. 来自玉米的P. asteris ' 菌株和 99.92% 和 99.55% 具有 'Ca. 分别与P. pruni ' 相关的菌株。RFLP 分析允许将这些植原体菌株包含在 16SrI-B 和 16SrIII-J 亚组中;然而,在某些情况下,这两种植原体存在于混合感染中。16SrIII-J 植原体首次被报道与玉米丛生矮化病有关,这代表了疾病流行病学在受影响地区进行适当管理的相关信息。

更新日期:2022-02-06
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