Etoposide (Eto) is an anti-cancer drug that is associated with serious adverse effects on male reproductive function. Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) and selenium (Se) are known as anti-inflammatory, anti-apoptotic and anti-oxidant agents. This work was designed to investigate changes in the biochemical parameters as well as alterations in Sertoli cell vimentin expression, ultrastructure and ectoplasmic specializations (ESs) following Eto treatment and to assess the ameliorative effect of ω-3 versus Se on these alterations. Eighty four adult male albino rats were used and classified into four groups: group I (control group), group II (Eto group) received Eto in a single intra-peritoneal (IP) dose (60 mg/kg B.W.), group III (Eto & ω-3 group) received the single IP dose of Eto as well as ω-3 (300 mg/kg B.W./day by intra-gastric intubation) starting 5 days before Eto injection till the time of sacrifice & group IV (Eto & Se group) received the single IP dose of Eto as well as Se (0.5 mg/kg B.W./day IP) starting 5 days before Eto injection till the time of sacrifice. The rats were subdivided into 2 subgroups (a) and (b) that were sacrificed 3 and 7 days after Eto injection respectively. Eto administration in group II induced increase in malondialdehyde (MDA), decrease in superoxide dismutase (SOD), collapse of Sertoli cell vimentin filaments and ultrastructural degenerative changes in both Sertoli cells and ESs. Se (group IV) reversed Eto toxic effects potently, while ω-3 (group III) had some limited protective effects.

依托泊苷 (Eto) 是一种抗癌药物，对男性生殖功能有严重的不良影响。Omega-3 多不饱和脂肪酸 (ω-3 PUFA) 和硒 (Se) 被称为抗炎剂、抗细胞凋亡剂和抗氧化剂。这项工作旨在研究 Eto 处理后生化参数的变化以及支持细胞波形蛋白表达、超微结构和外质特化 (ESs) 的变化，并评估 ω-3 与 Se 对这些变化的改善作用。使用 84 只成年雄性白化大鼠并将其分为四组：I 组（对照组）、II 组（Eto 组）单次腹膜内 (IP) 剂量（60 mg/kg BW）接受 Eto，III 组（ Eto＆ω-3组）从 Eto 注射前 5 天开始到处死时接受单次 IP 剂量的 Eto 以及 ω-3（300 mg/kg BW/天，通过胃内插管）& IV 组（Eto & Se 组）接受单次Eto 和 Se（0.5 mg/kg BW/天 IP）的 IP 剂量从 Eto 注射前 5 天开始直至处死。大鼠被分为 2 个亚组(a)和(b)分别在 Eto 注射后 3 天和 7 天处死。II 组中的 Eto 给药导致丙二醛 (MDA) 增加、超氧化物歧化酶 (SOD) 减少、支持细胞波形蛋白丝塌陷以及支持细胞和 ES 的超微结构退行性变化。Se（第 IV 组）有效逆转 Eto 毒性作用，而 ω-3（第 III 组）具有一些有限的保护作用。