Abstract

Extensive use of petroleum has caused many habitats to be contaminated in sea worldwide. Oil pollution impacts the environment and poses a direct or indirect health treats to different forms of life. In order to reduce the dangers and threats of these pollutions, it is important to choose a suitable method for purification and removal of petroleum compounds. There is an urgent need to clean up the waters with environmentally friendly and inexpensive methods. This study investigated the biodegradation of total petroleum hydrocarbons (TPH) using simultaneously Pseudomonas aeruginosa and Cladophora glomerata in southern Caspian Sea coastline. Coastline sediment samples were collected, with P. aeruginosa being the predominant strain. Afterwards gathered purified algae were placed in a lattice basket. Then bacteria and algae were cultured in triplicates in the presence of gasoline concentrations and under specific experimental conditions of varying temperature, pH, salinity, dissolved oxygen and incubation periods in aquariums of sea water. The data representing the gasoline biodegradation in the samples were statistically analyzed. In containing varying concentrations of gasoline and optimized experimental conditions for temperature, pH, salinity, dissolved oxygen and incubation period, maximum biodegradation of TPH was achieved by culturing P. aeruginosa strains and C. glomerata with the sea water samples of gasoline. Based on the results of statistical analysis the gram-negative bacteria, P. aeruginosa and the green algae C. glomerata almost completely biodegraded TPH contaminants from the samples’ culture media it was able to decompose almost 95% of crude oil over 28 days of incubation. It is concluded that the use of P. aeruginosa and C. glomerata together is an efficient method for the biodegradation of Caspian coastal waters contaminated with TPH.

中文翻译：

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里海海岸线水域铜绿假单胞菌和球毛藻同时去除石油污染物

摘要

石油的广泛使用已导致全球许多海洋栖息地受到污染。石油污染会影响环境，并对不同形式的生命造成直接或间接的健康影响。为了减少这些污染的危害和威胁，选择合适的石油化合物提纯和去除方法非常重要。迫切需要用环保和廉价的方法清理水域。本研究调查了在里海南部海岸线同时使用铜绿假单胞菌和球毛藻对总石油烃 (TPH) 的生物降解。收集了海岸线沉积物样品，铜绿假单胞菌成为主要菌株。然后将收集的纯化藻类放入格子篮中。然后，在存在汽油浓度的情况下，在海水水族馆中不同温度、pH、盐度、溶解氧和孵化期的特定实验条件下，一式三份培养细菌和藻类。对代表样品中汽油生物降解的数据进行了统计分析。在含有不同浓度的汽油和优化温度、pH、盐度、溶解氧和孵育期的实验条件下，通过培养铜绿假单胞菌菌株和球状假单胞菌实现了 TPH 的最大生物降解与汽油的海水样品。根据统计分析结果，革兰氏阴性菌、铜绿假单胞菌和绿藻C.glomerata几乎完全生物降解样品培养基中的 TPH 污染物，它能够在 28 天的孵化中分解几乎 95% 的原油. 得出的结论是，铜绿假单胞菌和C.glomerata一起使用是生物降解被 TPH 污染的里海沿海水域的有效方法。