Myrtle (Myrtus communis L.) is a typical plant cover of the Mediterranean region. It is an evergreen perennial shrub or small tree belonging to Myrtaceae family with a natural spread in tropical and subtropical areas. RNA sequencing technique is a common method used to identify transcriptomes. Next-generation sequencing technologies have recently been used for identification and development of SSR markers. This study was conducted to develop SSR markers in myrtle plants with the use of next-generation sequencing technologies. Illumina HiSeq 2000 sequencing device was used for RNA sequencing in two different myrtle genotypes (one with black fruits and the other one with white fruits). RNA-Seq analyses were conducted in two different myrtle genotypes and de novo analyses were performed to identify repeat regions of the genome. Present analyses revealed the greatest repeat pattern as 12,885 di-nucleotide. Number of repeat patterns varied between 12–11,776. Appropriate SSR primers able to amplify these repeat patterns were designed. Within the scope of this study, more than 12,000 SSR primers were developed.

桃金娘（Myrtus Communis  L.）是地中海地区典型的植物覆盖物。桃金娘科常绿多年生灌木或小乔木，自然分布于热带、亚热带地区。RNA测序技术是用于鉴定转录组的常用方法。新一代测序技术最近已被用于识别和开发 SSR 标记。本研究旨在利用下一代测序技术在桃金娘植物中开发 SSR 标记。Illumina HiSeq 2000 测序设备用于对两种不同的桃金娘基因型（一种带有黑色果实，另一种带有白色果实）进行 RNA 测序。RNA-Seq 分析在两种不同的桃金娘基因型和从头进行进行分析以确定基因组的重复区域。目前的分析显示最大的重复模式为 12,885 个二核苷酸。重复模式的数量在 12–11,776 之间变化。设计了能够放大这些重复模式的适当 SSR 引物。在本研究范围内，开发了 12,000 多个 SSR 引物。