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Molecular Characterization of Almond Cultivars Using Simple Sequence Repeat Markers
Erwerbs-Obstbau ( IF 1.3 ) Pub Date : 2022-02-02 , DOI: 10.1007/s10341-022-00640-7
Farhad Esgandaripirmorad 1 , Harun Karcı 1 , Aibibula Paizila 1 , Hayat Topçu 1 , Salih Kafkas 1
Affiliation  

Genetic diversity analysis of twenty-two almond cultivars originated from eight different countries was performed to determine genetic relationship and variation between them using simple sequence repeat (SSR) markers. A total of 28 SSR loci were chosen from 40 SSRs based on their polymorphism levels. A total of 265 alleles were generated from 28 SSR loci ranging from 2 to 14 alleles with an average of 9.46 per locus. According to genetic diversity analysis, the mean value of the effective number of alleles, observed heterozygosity and expected heterozygosity were calculated as 5.85, 0.75 and 0.76, respectively. The highest number of alleles was obtained from the CPDCT042 locus with 14 alleles. The highest expected heterozygosity (0.90) polymorphism information content values were obtained from the CPDCT042 locus. Additionally, 21 SSR loci produced specific alleles for 17 cultivars. The almond cultivars were separated into three main clusters based on UPGMA (unweighted pair group method with arithmetic averages), PCoA (principal component analysis) and STRUCTURE analysis. The cultivars originated from Turkey grouped in one cluster, while the other cultivars were in the remaining two clusters. The highest genetic distance was calculated between ‘Nurlu’ and ‘Cristomorto’ cultivars, while ‘Nonpareil’ and ‘Sonora’ were the closest ones. Consequently, the results in this study will provide useful information for fingerprinting of cultivars, future breeding and genetic studies in almond.



中文翻译:

使用简单序列重复标记对杏仁品种进行分子表征

对来自 8 个不同国家的 22 个杏仁品种进行遗传多样性分析,以使用简单序列重复 (SSR) 标记确定它们之间的遗传关系和变异。根据多态性水平,从 40 个 SSR 中选择了总共 28 个 SSR 位点。从 28 个 SSR 基因座产生了总共 265 个等位基因,范围从 2 到 14 个等位基因,每个基因座平均有 9.46 个。根据遗传多样性分析,计算出有效等位基因数、观察杂合度和预期杂合度的平均值分别为5.85、0.75和0.76。最高数量的等位基因来自具有 14 个等位基因的 CPDCT042 基因座。从 CPDCT042 基因座获得最高的预期杂合性 (0.90) 多态性信息含量值。此外,21 个 SSR 基因座产生了 17 个品种的特定等位基因。基于UPGMA(具有算术平均值的未加权配对组法)、PCoA(主成分分析)和STRUCTURE分析,将杏仁品种分为三个主要聚类。来自土耳其的品种被归为一个集群,而其他品种则在其余两个集群中。'Nurlu' 和'Cristomorto' 栽培品种之间的遗传距离最高,而'Nonpareil' 和'Sonora' 是最接近的。因此,本研究的结果将为杏仁品种的指纹识别、未来的育种和遗传研究提供有用的信息。PCoA(主成分分析)和结构分析。来自土耳其的品种被归为一个集群,而其他品种则在其余两个集群中。'Nurlu' 和'Cristomorto' 栽培品种之间的遗传距离最高,而'Nonpareil' 和'Sonora' 是最接近的。因此,本研究的结果将为杏仁品种的指纹识别、未来的育种和遗传研究提供有用的信息。PCoA(主成分分析)和结构分析。来自土耳其的品种被归为一个集群,而其他品种则在其余两个集群中。'Nurlu' 和'Cristomorto' 栽培品种之间的遗传距离最高,而'Nonpareil' 和'Sonora' 是最接近的。因此,本研究的结果将为杏仁品种的指纹识别、未来的育种和遗传研究提供有用的信息。

更新日期:2022-02-03
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