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Transcriptome and co-expression network analyses provide insights into fruit shading that enhances carotenoid accumulation in pomelo (Citrus grandis)
Horticultural Plant Journal ( IF 5.7 ) Pub Date : 2022-01-31 , DOI: 10.1016/j.hpj.2022.01.007
Qihang Jiang 1 , Junli Ye 1 , Kaijie Zhu 1 , Fangfang Wu 2 , Lijun Chai 1 , Qiang Xu 1 , Xiuxin Deng 1
Affiliation  

Carotenoids are indispensable for human health, and citrus fruit are a crucial source of dietary carotenoids. Bagging, an important orchard practice to enhance fruit economic value, is widely used in many horticultural crops, including citrus fruit. The bagged ‘Majiayou’ pomelo (Citrus grandis) produces vivid deeper red pulp, a fantastic agronomic trait, but the underlying molecular regulatory mechanism remains largely unexplored. Here, the enhancement of carotenoids, especially lycopene, was confirmed by HPLC analysis of carotenoids in the pulp of bagged fruit and controls. qRT-PCR analysis of the 14 carotenoid pathway genes further revealed that upregulated PSY and downregulated CCD1 expression in bagged fruit could directly enhance the accumulation of carotenoids. In addition, RNA-seq analysis identified 311 differentially expressed genes (DEGs) in the bagged fruit and controls in five developmental stages. Weighted gene co-expression network analysis (WGCNA) identified 13 critical candidate genes among the DEGs, which are closely associated with lycopene accumulation. The underlying regulatory mechanism of these candidate genes on the transcription of carotenoid pathway genes in the bagged fruit was discussed. Considering that the candidate genes were involved in the corresponding metabolic pathways, the increase in sucrose content and decrease in ABA in bagged fruit were also identified, implying that these candidate genes may be indirectly related to carotenoid enhancement in pulp by regulating phytohormones, primary metabolism, and stress responses. The results provide new insights into the potential regulatory mechanism of lycopene enhancement in the pulp of bagged ‘Majiayou’ pomelo, facilitating breeding and orchard management efforts to improve the nutritional quality and esthetic value of citrus, and perhaps other fruit crops.



中文翻译:

转录组和共表达网络分析提供了对增强柚子(Citrus grandis)中类胡萝卜素积累的水果阴影的见解

类胡萝卜素对人类健康是必不可少的,柑橘类水果是膳食类胡萝卜素的重要来源。套袋是提高水果经济价值的重要果园做法,广泛用于包括柑橘类水果在内的许多园艺作物。袋装的“马家友”柚(Citrus grandis)产生鲜艳的深红色果肉,这是一种奇妙的农艺性状,但潜在的分子调控机制在很大程度上仍未被探索。在这里,通过对袋装水果和对照果肉中类胡萝卜素的 HPLC 分析证实了类胡萝卜素,尤其是番茄红素的增强。对 14 种类胡萝卜素途径基因的 qRT-PCR 分析进一步揭示了上调PSY和下调CCD1在袋装水果中的表达可以直接促进类胡萝卜素的积累。此外,RNA-seq 分析在袋装水果和五个发育阶段的对照中鉴定了 311 个差异表达基因 (DEG)。加权基因共表达网络分析 (WGCNA) 确定了 DEG 中的 13 个关键候选基因,这些基因与番茄红素积累密切相关。讨论了这些候选基因对袋装果实中类胡萝卜素途径基因转录的潜在调控机制。考虑到候选基因参与了相应的代谢途径,袋装果实中蔗糖含量的增加和ABA的降低也被鉴定出,这意味着这些候选基因可能与通过调节植物激素间接增加果肉中的类胡萝卜素有关,初级代谢和应激反应。该结果为袋装“马家优”柚果肉中番茄红素增强的潜在调控机制提供了新的见解,促进了育种和果园管理工作,以提高柑橘和其他水果作物的营养质量和审美价值。

更新日期:2022-01-31
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