This study contains intravital microscopy (IVM) data to characterize organ scaffolds created using decellularization. Decellularization creates cell-free collagen-based matrices from native organs, which can be used as scaffolds for regenerative medicine applications. This data set contains in vivo assays evaluating the effectiveness of decellularization and structural and functional integrity of the acellular nephron in the post-transplantation environment. Qualitative and quantitative assessments of scaffold DNA concentrations, tissue fluorescence signals, structural and functional integrities of various decellularized nephron segments, and velocity within the microcirculation were acquired and compared to the native (non-transplanted) organ. Cohorts of 2-3-month-old male Sprague Dawley rats were used: non-transplanted (n = 4), transplanted day 0 (n=4), transplanted day 1 (n = 4), transplanted day 2 (n = 4), and transplanted day 7 (n = 4). OIF formatted files illustrate IVM measurement processes and are made publicly available in a data repository. Such data is intended to support scientific reproducibility, complement future renal studies, and extend the use of this powerful imaging application to analyze other organ scaffold systems.

中文翻译：

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使用活体显微镜进行器官支架表征

本研究包含活体显微镜 (IVM) 数据，以表征使用脱细胞化创建的器官支架。去细胞化从天然器官中产生基于胶原蛋白的无细胞基质，可用作再生医学应用的支架。该数据集包含体内评估去细胞化的有效性以及脱细胞肾单位在移植后环境中的结构和功能完整性的测定。对支架 DNA 浓度、组织荧光信号、各种脱细胞肾单位片段的结构和功能完整性以及微循环内的速度进行定性和定量评估，并与天然（非移植）器官进行比较。使用 2-3 个月大的雄性 Sprague Dawley 大鼠群组：未移植 (n = 4)、移植第 0 天 (n = 4)、移植第 1 天 (n = 4)、移植第 2 天 (n = 4) )，移植第 7 天 (n = 4)。OIF 格式的文件说明了 IVM 测量过程，并在数据存储库中公开提供。此类数据旨在支持科学可重复性，