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Comparison Between Franz Diffusion Cell and a novel Micro-physiological System for In Vitro Penetration Assay Using Different Skin Models.
SLAS Technology: Translating Life Sciences Innovation ( IF 2.7 ) Pub Date : 2022-01-02 , DOI: 10.1016/j.slast.2021.12.006
Ilaria Pulsoni 1 , Markus Lubda 2 , Maurizio Aiello 3 , Arianna Fedi 4 , Monica Marzagalli 1 , Joerg von Hagen 2 , Silvia Scaglione 3
Affiliation  

In vitro diffusive models are an important tool to screen the penetration ability of active ingredients in various formulations. A reliable assessment of skin penetration enhancing properties, mechanism of action of carrier systems, and an estimation of a bioavailability are essential for transdermal delivery. Given the importance of testing the penetration kinetics of different compounds across the skin barrier, several in vitro models have been developedThe aim of this study was to compare the Franz Diffusion Cell (FDC) with a novel fluid-dynamic platform (MIVO) by evaluating penetration ability of caffeine, a widely used reference substance, and LIP1, a testing molecule having the same molecular weight but a different lipophilicity in the two diffusion chamber systems. A 0.7% caffeine or LIP1 formulation in either water or propylene glycol (PG) containing oleic acid (OA) was topically applied on the Strat-M® membrane or pig ear skin, according to the infinite-dose experimental condition (780 ul/cm2). The profile of the penetration kinetics was determined by quantify the amount of molecule absorbed at different time-points (1, 2, 4, 6, 8 hours), by means of HPLC analysis. Both diffusive systems show a similar trend for caffeine and LIP1 penetration kinetics. The Strat-M® skin model shows a lower barrier function than the pig skin biopsies, whereby the PGOA vehicle exhibits a higher penetration, enhancing the effect for both diffusive chambers and skin surrogates. Most interestingly, MIVO diffusive system better predicts the lipophilic molecules (i.e. LIP1) permeation through highly physiological fluid flows resembled below the skin models.

中文翻译:

Franz 扩散池与使用不同皮肤模型进行体外渗透测定的新型微生理系统的比较。

体外扩散模型是筛选各种制剂中活性成分渗透能力的重要工具。对皮肤渗透增强特性、载体系统的作用机制和生物利用度的估计的可靠评估对于透皮给药是必不可少的。鉴于测试不同化合物穿过皮肤屏障的渗透动力学的重要性,已经开发了几种体外模型本研究的目的是通过评估渗透来比较弗朗兹扩散池 (FDC) 与新型流体动力学平台 (MIVO)咖啡因(一种广泛使用的参考物质)和 LIP1(一种具有相同分子量但在两个扩散室系统中的亲脂性不同的测试分子)的能力。一个 0。根据无限剂量实验条件 (780 ul/cm2),在水或含有油酸 (OA) 的丙二醇 (PG) 中的 7% 咖啡因或 LIP1 配方局部应用于 Strat-M® 膜或猪耳朵皮肤. 通过 HPLC 分析,通过量化在不同时间点(1、2、4、6、8 小时)吸收的分子量来确定渗透动力学曲线。两种扩散系统都显示出咖啡因和 LIP1 渗透动力学的相似趋势。Strat-M® 皮肤模型显示出比猪皮肤活检更低的屏障功能,因此 PGOA 载体表现出更高的渗透性,增强了扩散室和皮肤替代物的效果。最有趣的是,MIVO 扩散系统更好地预测了亲脂性分子(即
更新日期:2022-01-02
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